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Differentiation of cotton-defoliating and nondefoliating pathotypes of Verticillium dahliae by RAPD and specific PCR analyses

AuthorsPérez-Artés, Encarnación ; García-Pedrajas, María D.; Bejarano-Alcázar, J.; Jiménez-Díaz, R. M.
KeywordsVerticillium wilt
Gossypium hirsutum
molecular markers
genetic diversity
Issue Date2000
PublisherKluwer Academic Publishers
CitationMarilo, 1
AbstractSevere Verticillium wilt of cotton in southern Spain is associated with the spread of a highly virulent, defoliating (D) pathotype of Verticillium dahliae. Eleven of the D and 15 of a mildly virulent, nondefoliating (ND) pathotype were analyzed by random amplified polymorphic DNA (RAPD) using the polymerase chain reaction (PCR). Six of 21 primers tested generated pathotype-associated RAPD bands.
Publisher version (URL)http://www.springerlink.com/content/mt5v1jr13uu18x05/fulltext.pdf
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