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Título: | Isolation and characterization of tyramine-producing Enterococcus faecium strains from red wine |
Autor: | Capozzi, Vittorio; Ladero Losada, Víctor Manuel CSIC ORCID ; Beneduce, Luciano; Fernández García, María CSIC ORCID ; Álvarez González, Miguel Ángel CSIC ORCID ; Bernoit, Bach; Laurent, Barnavon; Grieco, F.; Spano, Giuseppe | Fecha de publicación: | may-2011 | Editor: | Academic Press | Citación: | Food Microbiology 28(3): 434-439 (2011) | Resumen: | Enterococcus faecium strains were isolated from red wines undergoing malolactic fermentation and identified by comparison of their 16S rDNA gene sequences with those included in the GenEMBL Databases. The tyrosine decarboxylase gene was identified in all the strains analysed by PCR using gene-specific primers and the ability to produce tyramine in a synthetic media was analysed by RP-HPLC. Survival of an . E. faecium strain was also evaluated in microvinification assays using two different musts with different ethanol concentrations (10% and 12% (v/v)). Tyramine production was monitored during the vinification trials. Our results suggest that . E. faecium strains isolated from wine are able to produce tyramine and tolerate wine conditions following a pre-acidic stress. © 2010 Elsevier Ltd. | URI: | http://hdl.handle.net/10261/81011 | DOI: | 10.1016/j.fm.2010.10.005 | Identificadores: | doi: 10.1016/j.fm.2010.10.005 issn: 0740-0020 |
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