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Título : Towards positional isolation of three quantitative trait Loci conferring resistance to powdery mildew in two Spanish barley landraces
Autor : Silvar Casao, Cristina ; Perovic, Dragan; Nussbaumer, Thomas; Spannagl, Manuel; Usadel, Björn; Casas Cendoya, Ana María ; Igartua Arregui, Ernesto ; Ordon, Frank
Palabras clave : Barley
Basic leucine zipper motif
Chromosome
Chromosome 6h
Chromosome 7h
Controlled study
Gene identification
Genetic association
Genetic marker
Genome analysis
Nonhuman
Plant defense
Plant genome
Plant pathogen interaction
Powdery mildew
Protein function
Quantitative trait locus
Sequence homology
Fecha de publicación : 24-jun-2013
Editor: Public Library of Science
Citación : Silvar C, Perovic D, Nussbaumer T, Spannagl M, Usadel B, Casas A, Igartua E, Ordon F. Towards positional isolation of three quantitative trait Loci conferring resistance to powdery mildew in two Spanish barley landraces. PLoS ONE 8 (6): e67336 (2013)
Resumen: Three quantitative trait loci (QTL) conferring broad spectrum resistance to powdery mildew, caused by the fungus Blumeria graminis f. sp. hordei, were previously identified on chromosomes 7HS, 7HL and 6HL in the Spanish barley landrace-derived lines SBCC097 and SBCC145. In the present work, a genome-wide putative linear gene index of barley (Genome Zipper) and the first draft of the physical, genetic and functional sequence of the barley genome were used to go one step further in the shortening and explicit demarcation on the barley genome of these regions conferring resistance to powdery mildew as well as in the identification of candidate genes. First, a comparative analysis of the target regions to the barley Genome Zippers of chromosomes 7H and 6H allowed the development of 25 new gene-based molecular markers, which slightly better delimit the QTL intervals. These new markers provided the framework for anchoring of genetic and physical maps, figuring out the outline of the barley genome at the target regions in SBCC097 and SBCC145. The outermost flanking markers of QTLs on 7HS, 7HL and 6HL defined a physical area of 4 Mb, 3.7 Mb and 3.2 Mb, respectively. In total, 21, 10 and 16 genes on 7HS, 7HL and 6HL, respectively, could be interpreted as potential candidates to explain the resistance to powdery mildew, as they encode proteins of related functions with respect to the known pathogen defense-related processes. The majority of these were annotated as belonging to the NBS-LRR class or protein kinase family. © 2013 Silvar et al.
Descripción : 12 Pag., 3 Fig., 3 Tabl. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Versión del editor: http://dx.doi.org/10.1371/journal.pone.0067336
URI : http://hdl.handle.net/10261/80818
DOI: 10.1371/journal.pone.0067336
Identificadores: doi: 10.1371/journal.pone.0067336
issn: 1932-6203
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