Por favor, use este identificador para citar o enlazar a este item:
http://hdl.handle.net/10261/80640
COMPARTIR / EXPORTAR:
SHARE CORE BASE | |
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE | |
Título: | CSF Presenilin-1 complexes are increased in Alzheimer’s disease |
Autor: | García-Ayllón, María-Salud CSIC ORCID; Campanari, María Letizia CSIC; Brinkmalm, Gunnar; Rábano, Alberto; Alom, Jordi; Saura, Carlos A.; Andreasen, Niels; Blennow, Kaj; Sáez-Valero, Javier CSIC ORCID | Palabras clave: | Alzheimer’s disease Cerebrospinal fluid Diagnostic Biomarkers Presenilin 1 |
Fecha de publicación: | 6-ago-2013 | Editor: | BioMed Central | Citación: | Acta Neuropathologica Communications 1(1) : 46- (2013) | Resumen: | Background Presenilin-1 (PS1) is the active component of the amyloid precursor protein cleaving γ-secretase complex. PS1 protein is a transmembrane protein containing multiple hydrophobic regions which presence in cerebrospinal fluid (CSF) has not been measured to date. This study assesses whether PS1 and other components of the γ-secretase complex are present in CSF. Results Here, we show that PS1 is present in ventricular post-mortem and lumbar ante-mortem CSF, and plasma as 100–150-kDa hetero-complexes containing both the N- and C-terminal fragments (NTF and CTF) of the protein. Immunoprecipitation and immunoblotting with different antibodies confirmed the identity of the PS1 species. The γ-secretase components, APH-1 (anterior pharynx-defective 1) and PEN-2 (presenilin enhancer 2), as well as presenilin-2 (PS2) fragments, co-exist within these CSF complexes, while nicastrin is not detected. These CSF-PS1 complexes differ from active γ-secretase membrane-complexes, and may represent nonspecific aggregation of the PS1 protein. Levels of PS1 complexes are increased in CSF samples from autopsy-confirmed Alzheimer’s disease (AD) cases and were found to be more stable than complexes in CSF from control subjects. Despite similar levels of total PS1 in CSF from probable AD patients and cognitively normal subjects, an increased proportion of highly stable PS1 complexes were observed in AD CSF. Conclusions Our data suggest that fragments of the PS1 protein present in CSF as complexes may be useful as a biomarker for AD. | Versión del editor: | http://dx.doi.org/10.1186/2051-5960-1-46 | URI: | http://hdl.handle.net/10261/80640 | DOI: | 10.1186/2051-5960-1-46 | Identificadores: | 10.1186/2051-5960-1-46 |
Aparece en las colecciones: | (IN) Artículos |
Ficheros en este ítem:
Fichero | Descripción | Tamaño | Formato | |
---|---|---|---|---|
2051-5960-1-46.xml | 94,13 kB | XML | Visualizar/Abrir | |
2051-5960-1-46.pdf | 1,94 MB | Adobe PDF | Visualizar/Abrir |
CORE Recommender
PubMed Central
Citations
15
checked on 06-abr-2024
SCOPUSTM
Citations
20
checked on 09-abr-2024
WEB OF SCIENCETM
Citations
19
checked on 27-feb-2024
Page view(s)
358
checked on 19-abr-2024
Download(s)
424
checked on 19-abr-2024