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Title: Synthesis of methyl Alpha-D-Glucoolicosaccharides by Entrapped Dextransucrase from Leuconostoc mesenteroides B-1299
Authors: Gómez de Segura, María Aránzazu, Alcalde Galeote, Miguel, Bernabé, M., Ballesteros, Antonio, Plou Gasca, Francisco José
Keywords: Glucansucrases
Acceptor reaction
Methyl Polyglucosides
Encapsulation
Alginate
Issue Date: 2006
Publisher: Elsevier
Abstract: The synthesis of methyl alpha-D-glucooligosaccharides, using sucrose as glucosyl donor and methyl alpha-D-glucopyranoside as acceptor, was studied with dextransucrase from Leuconostoc mesenteroides NRRL B-1299. The enzyme was immobilized by entrapment in alginate. By NMR and mass spectrometry we identified three homologous series (S1-S3) of methyl alpha-D-glucooligosaccharides. Series S2 and S3 were characterized by the presence of alpha(1-2) linkages, in combination with alpha(1-6) bonds. Two parameters, sucrose to acceptor concentration ratio (S/A) and the total sugar concentration (TSC) determined the yield of methyl alpha-D-glucooligosaccharides. The maximum concentration achieved of the first acceptor product, methyl alpha-D-isomaltoside, was 65 mM using a S/A 1:4 and a TSC of 336 g l-1. When increasing temperature, a shift of selectivity towards compounds containing alpha(1-2) bonds was observed. The formation of leucrose as a side process was very significant (reaching values of 32 g l-1) at high sucrose concentrations.
Publisher version (URL): doi:10.1016/j.jbiotec.2005.12.031
URI: http://hdl.handle.net/10261/8060
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Citation: Journal of Biotechnology 124, 439-445 (2006)
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