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Analysis of the gonadal transcriptome during sex determination, sex differentiation and gonadal maturation in the sea bass (Dicentrarchus labrax) and turbot (Scophthalmus maximus) by 454 sequencing and two specific oligo-based microarrays

AuthorsRibas, Laia ; Crespo, Berta ; Díaz, Noelia ; Gómez, Ana ; Pardo, Belén G.; Reinhardt, Richard; MacKenzie, Simon; Martínez, Paulino; Zanuy, Silvia ; Piferrer, Francesc
Issue DateOct-2009
CitationNext Generation Sequencing 2009 International Meeting (2009)
AbstractFish represent unique models to study vertebrate sex determination and differentiation, as well as gonad maturation for several reasons. First, fish are by far the most abundant type of vertebrates and exhibit all reproduction types known in vertebrates (gonochorism, hermaphroditism and unisexuality). Furthermore, the processes of sex determination, differentiation and gonad maturation are the result of complex genetic, environmental and social interactions. Thus, for example, temperature or population density can easily influence the course of sex differentiation, a phenomenon that represents one of the most dramatic examples of phenotypic plasticity. The gonads are then the only organ in vertebrates that starting from common undifferentiated rudiment can undergo two completely different developmental pathways, resulting in a testis or ovary, and the environmental conditions imposed by modern farming provide an excellent framework where to study these essential biological processes. We are applying next generation sequencing technologies, specifically 454 sequencing, to obtain a detailed overview of both the European sea bass (Dicentrarchus labrax) and turbot (Scophthalmus maximus) gonadal transcriptome under a variety of developmental and experimental conditions. In addition, oligo-based microarrays are being built enriched with genes expressed in the reproductive axis with the purpose of discovering new genes and signaling routes
DescriptionNext Generation Sequencing 2009 International Meeting, 1-3 Octubre 2009, Barcelona
Appears in Collections:(IATS) Comunicaciones congresos
(ICM) Comunicaciones congresos
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