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Open Access item On the transfructosylation activity and selectivity of microbial beta-fructofuranosidases for the production of prebiotics

Authors:Fernández-Arrojo, Lucía
Alvaro-Benito, Miguel
Ghazi, Iraj
Abreu Felipe, Miguel A. de
Linde López, Dolores
Gutiérrez Alonso, Patricia
Alcalde Galeote, Miguel
Jiménez-Barbero, Jesús
Jiménez Díaz, Antonio
Ballesteros, Antonio O.
Fernández Lobato, María
Plou, Francisco J.
Issue Date:2007
Citation:Journal of Biotechnology 131S S107 (2007)
Abstract:We have purified a fructosyltransferase from Aspergillus aculeatus present in commercial samples of Pectinex Ultra SP-L. We have also isolated and purified extracellular beta-fructofuranosidases from cultures of the yeasts Xanthophyllomyces dendrorhous (formerly Phaffia rhodozyma, Schwanniomyces occidentalis and Rhodotorula gracilis. The transferase activity of these enzymes was analysed in detail, along with that of beta-fructofuranosidase from Saccharomyces cerevisiae. Interestingly, the maximum FOS yield (expressed as weight percentage of the total amount of carbohydrates in the mixture) was significantly higher with the enzyme from A. aculeatus (61%), compared with those from X. dendrorhous, S. occidentalis and R. gracilis (17-19 %), and that from S. cerevisiae (8%). In addition, significant differences were encountered in the selectivity of the process: A. aculeatus synthesized only 1F-FOS, X. dendrorhous formed basically neo-kestose, whereas S. occidentalis and S. cerevisiae were selective towards the production of 6-kestose. R. gracilis synthesized a complex mixture of di-, tri- and tetrasaccharides.
Publisher version (URL):http://dx.doi.org/10.1016/j.jbiotec.2007.07.184
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