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Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/8033
Title: On the transfructosylation activity and selectivity of microbial beta-fructofuranosidases for the production of prebiotics
Authors: Fernández Arrojo, Lucía ; Álvaro-Benito, Miguel; Ghazi, Iraj ; Abreu Felipe, Miguel A. de; Linde López, Dolores; Gutiérrez Alonso, Patricia; Alcalde Galeote, Miguel ; Jiménez-Barbero, Jesús; Jiménez Díaz, Antonio; Ballesteros Olmo, Antonio ; Fernández Lobato, María; Plou Gasca, Francisco José
Issue Date: 2007
Publisher: Elsevier
Citation: Journal of Biotechnology 131S S107 (2007)
Abstract: We have purified a fructosyltransferase from Aspergillus aculeatus present in commercial samples of Pectinex Ultra SP-L. We have also isolated and purified extracellular beta-fructofuranosidases from cultures of the yeasts Xanthophyllomyces dendrorhous (formerly Phaffia rhodozyma, Schwanniomyces occidentalis and Rhodotorula gracilis. The transferase activity of these enzymes was analysed in detail, along with that of beta-fructofuranosidase from Saccharomyces cerevisiae. Interestingly, the maximum FOS yield (expressed as weight percentage of the total amount of carbohydrates in the mixture) was significantly higher with the enzyme from A. aculeatus (61%), compared with those from X. dendrorhous, S. occidentalis and R. gracilis (17-19 %), and that from S. cerevisiae (8%). In addition, significant differences were encountered in the selectivity of the process: A. aculeatus synthesized only 1F-FOS, X. dendrorhous formed basically neo-kestose, whereas S. occidentalis and S. cerevisiae were selective towards the production of 6-kestose. R. gracilis synthesized a complex mixture of di-, tri- and tetrasaccharides.
Publisher version (URL): http://dx.doi.org/10.1016/j.jbiotec.2007.07.184
URI: http://hdl.handle.net/10261/8033
DOI: 10.1016/j.jbiotec.2007.07.184
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