Por favor, use este identificador para citar o enlazar a este item: http://hdl.handle.net/10261/8021
COMPARTIR / EXPORTAR:
logo share SHARE logo core CORE BASE
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE

Invitar a revisión por pares abierta
Título

Purification and kinetic characterization of a fructosyltransferase from Aspergillus aculeatus

AutorGhazi, Iraj CSIC; Fernández Arrojo, Lucía; García-Arellano, Humberto; Ferrer, Manuel CSIC ORCID; Ballesteros Olmo, Antonio CSIC ORCID; Plou Gasca, Francisco José CSIC ORCID
Palabras claveBeta-Fructofuranosidase
Prebiotics
Fructo-oligosaccharides
Fos
Nutraceuticals
Functional foods
Fecha de publicación2007
EditorElsevier
CitaciónJournal of Biotechnology Volume 128, Issue 1, 30 January 2007, Pages 204–211
ResumenA fructosyltransferase present in Pectinex Ultra SP-L, a commercial enzyme preparation from Aspergillus aculeatus, was purified to 107-fold and further characterised. The enzyme was a dimeric glycoprotein (20% w/w carbohydrate content) with a molecular mass of around 135 kDa for the dimer. Optimal activity/stability was found in the pH range 5.0-7.0 and at 60ºC. It was stable or slightly activated (up to 1.4-fold) in the presence of reducing agents such as dithiotreitol and 2-mercaptoethanol, and detergents such as sodium dodecylsulphate and Tween 80. The enzyme was able to transfer fructosyl groups from sucrose as donor producing the corresponding series of fructooligosaccharides: 1-kestose, nystose and fructosylnystose. Using sucrose as substrate, the kcat and Km values for transfructosylating activity were 1.62  0.09 104 s-1 and 0.53  0.05 M, whereas for hydrolytic activity the corresponding values were 775  25 s-1 and 27  3 mM. At elevated sucrose concentrations, the fructosyltransferase from A. aculeatus showed a high transferase/hydrolase ratio that confers it a great potential for the industrial production of prebiotic fructooligosaccharides.
Versión del editorhttp://dx.doi.org/10.1016/j.jbiotec.2006.09.017
URIhttp://hdl.handle.net/10261/8021
DOI10.1016/j.jbiotec.2006.09.017
Aparece en las colecciones: (ICP) Artículos




Ficheros en este ítem:
Fichero Descripción Tamaño Formato
postprintJBIOTEC.pdf321,79 kBAdobe PDFVista previa
Visualizar/Abrir
Mostrar el registro completo

CORE Recommender

SCOPUSTM   
Citations

131
checked on 15-mar-2024

WEB OF SCIENCETM
Citations

121
checked on 26-feb-2024

Page view(s)

455
checked on 19-mar-2024

Download(s)

1.118
checked on 19-mar-2024

Google ScholarTM

Check

Altmetric

Altmetric


NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.