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Campo DC | Valor | Lengua/Idioma |
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dc.contributor.author | Soriano Castán, Mercedes | - |
dc.contributor.author | Cistué Sola, Luis | - |
dc.contributor.author | Vallés Brau, María Pilar | - |
dc.contributor.author | Castillo Alonso, Ana María | - |
dc.date.accessioned | 2008-10-24T15:46:47Z | - |
dc.date.available | 2008-10-24T15:46:47Z | - |
dc.date.issued | 2007-12 | - |
dc.identifier.citation | Plant Cell Tissue Organ Culture Vol 91:225-234 | en_US |
dc.identifier.issn | 0167-6857 | - |
dc.identifier.uri | http://hdl.handle.net/10261/7990 | - |
dc.description | The original version is available at http://www.springerlink.com/content/l0788jw70644 | en_US |
dc.description.abstract | The aim of this work was to study the effects of colchicine application on chromosome doubling and androgenic response in anther and microspore culture of different bread wheat genotypes. Colchicine was applied during a mannitol stress pretreatment or during the first 48 h of culture at concentrations of 0, 150 and 300 mg l−1. When colchicine was applied during stress pretreatment, the percentage of doubling depended on genotype and concentration. A significant increase in doubling was observed with 300 mg l−1 in the low androgenic responding cv. Caramba. Colchicine incorporation during the first hours of culture improved percentage of doubling in all genotypes, in both anther and microspore culture. Application of 300 mg l−1 colchicine improved the percentage of doubling in the two low responding genotypes, to 118% of control in DH24033, and 75% in Caramba in microspore and anther culture, respectively. Concerning the androgenic response, the effect of colchicine on embryo formation and percentage of green plants depended on the genotype and on the culture method. In cv. Pavon, a 2- and a 3-fold increase in percentage of embryogenesis and green plants, respectively, were obtained with 300 mg l−1 colchicine in microspore culture. However, no significant differences in these two variables were observed in anther culture. The number of green doubled haploid (DH) plants reflects the index of success of the procedure. Regardless of the culture method, when colchicine was incorporated during the first hours of culture, the number of green DH plants increased significantly in three of four genotypes. These results confirm the usefulness of colchicine application during the first hours of culture in wheat breeding programs. | en_US |
dc.description.sponsorship | MS was the recipient of a fellowship from the Ministerio de Educación y Ciencia of the Spanish Government. We thank the Instituto de Agrobiotecnología y Recursos Naturales (UPNA-CSIC) for the use of the PA flow cytometer. The research was supported by Proyects from the Plan Nacional de Recursos y Tecnologías Agroalimentarias AGL2002-04139-C02-02 and AGL2005-07195-C02-01. | en_US |
dc.format.extent | 206515 bytes | - |
dc.format.mimetype | application/pdf | - |
dc.language.iso | eng | en_US |
dc.publisher | Springer Nature | en_US |
dc.rights | openAccess | en_US |
dc.subject | Doubled haploid | en_US |
dc.subject | Chromosome doubling | en_US |
dc.subject | Androgenesis | en_US |
dc.subject | Poaceae | en_US |
dc.subject | Stress pretreatment | en_US |
dc.title | Effects of colchicine on anther and microspore culture of bread wheat (Triticum aestivum L.) | en_US |
dc.type | artículo | en_US |
dc.description.peerreviewed | Peer reviewed | en_US |
dc.relation.publisherversion | http://www.springerlink.com/content/c02465422255n477/fulltext.pdf | en_US |
dc.identifier.e-issn | 1573-5044 | - |
dc.type.coar | http://purl.org/coar/resource_type/c_6501 | es_ES |
item.languageiso639-1 | en | - |
item.fulltext | With Fulltext | - |
item.openairecristype | http://purl.org/coar/resource_type/c_18cf | - |
item.cerifentitytype | Publications | - |
item.grantfulltext | open | - |
item.openairetype | artículo | - |
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Effects of colchicine on anther and microspore culture of bread wheat.pdf | 201,67 kB | Adobe PDF | Visualizar/Abrir |
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