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Título : Antioxidant activity of Haematococcus pluvialis cells grown in continuous culture as a function of their carotenoid and fatty acid content
Autor : Cerón, M. Carmen, García-Malea, María del Carmen, Rivas, Joaquín, Ancien Fernández, Francisco Gabriel, Fernández, José María, Del Rio, E., García Guerrero, Miguel, Molina-Grima, E.
Palabras clave : Haematococcus
Antioxidant
Fatty acid
Carotenoids
Astaxanthin
Fecha de publicación : 15-Dec-2006
Editor: Springer
Resumen: The influence of culture conditions on the quality of Haematococcus pluvialis biomass is assessed. Continuously grown cells have been characterised with respect to their astaxanthin, fatty acid content, and antioxidant activity and compared with those of non-growing haematocysts. Moderate limitation of nitrate availability (1.7 mM) under continuous growth conditions favoured the production of reddish palmelloid cells whose extracts possessed antioxidant activity equivalent to that of haematocyst extracts, despite the lower astaxanthin content (0.6%d.wt.), which is compensated by a higher fatty acid level (7.6%d.wt.). Green cells produced under nitrate saturation conditions (>4.7 mM) exhibit only 40% antioxidant activity than palmelloid. In addition, the major fatty acid present in palmelloid cells was oleic acid (40%f.a.), whereas, in both green cells and haematocysts, the main fatty acids were myristic, palmitic, and oleic acid (20–30%f.a. each). Biomass extracts were fractionated and analysed. The antioxidant capacity was a function of both the carotenoid and the fatty acid profiles, the antioxidant capacity of astaxanthin diesters fraction being 60% higher than astaxanthin monoesters fraction and twice than free astaxanthin. In such a way, the evaluation of the quality of H. pluvialis biomass must take into account both variables. When considering the production of H. pluvialis biomass for human consumption, special attention should be paid to the one-step continuous system approach for the generation of cells rich in both astaxanthin and fatty acids, as they have high antioxidant activity but without thick hard cell wall.
Descripción : Applied Microbiology and Biotechnology 74(5): 1112-1119 (2007)
Versión del editor: http://dx.doi.org/10.1007/s00253-006-0743-5
URI : http://hdl.handle.net/10261/7979
ISSN: 1432-0614 (Online)
DOI: 10.1007/s00253-006-0743-5
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