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dc.contributor.author | Díaz, Margarita | - |
dc.contributor.author | Sevillano, Laura | - |
dc.contributor.author | Rico, Sergio | - |
dc.contributor.author | Lombó, Felipe | - |
dc.contributor.author | Braña, Alfredo F. | - |
dc.contributor.author | Salas, Jose A | - |
dc.contributor.author | Méndez, Carmen | - |
dc.contributor.author | Santamaría, Ramón I. | - |
dc.date.accessioned | 2013-07-11T13:08:22Z | - |
dc.date.available | 2013-07-11T13:08:22Z | - |
dc.date.issued | 2013 | - |
dc.identifier | doi: 10.1111/1574-6968.12098 | - |
dc.identifier | issn: 0378-1097 | - |
dc.identifier | e-issn: 1574-6968 | - |
dc.identifier.citation | FEMS Microbiology Letters 342(2): 123-129 (2013) | - |
dc.identifier.uri | http://hdl.handle.net/10261/79479 | - |
dc.description.abstract | Phosphate metabolism regulates most of the life processes of microorganisms. In the present work we obtained and studied a Streptomyces lividans ppk/pstS double mutant, which lacks polyphosphate kinase (PPK) and the high-affinity phosphate-binding protein (PstS), impairing at the same time the intracellular storage of polyphosphate and the intake of new inorganic phosphate from a phosphate-limited medium, respectively. In some of the aspects analyzed, the ppk/pstS double mutant was more similar to the wt strain than was the single pstS mutant. The double mutant was thus able to grow in phosphate-limited media, whereas the pstS mutant required the addition of 1 mM phosphate under the assay conditions used. The double mutant was able to incorporate more than one fourth of the inorganic phosphate incorporated by the wt strain, whereas phosphate incorporation was almost completely impaired in the pstS mutant. Noteworthy, under phosphate limitation conditions, the double ppk/pstS mutant showed a higher production of the endogenous antibiotic actinorhodin and the heterologous antitumor 8-demethyl-tetracenomycin (up to 10-fold with respect to the wt strain), opening new possibilities for the use of this strain in the heterologous expression of antibiotic pathways. © 2013 Federation of European Microbiological Societies. | - |
dc.description.sponsorship | This work has been supported by grants BFU2006-13668 and EUI2008-03631 to R. I. Santamaría from the Ministerio de Educación y Ciencia. The IBFG acknowledges the institutional support of the Ramón Areces Foundation during 2011–2012. | - |
dc.language.iso | eng | - |
dc.publisher | Wiley-Blackwell | - |
dc.relation.isversionof | Postprint | - |
dc.rights | openAccess | - |
dc.title | High level of antibiotic production in a double polyphosphate kinase and phosphate-binding protein mutant of streptomyces lividans | - |
dc.type | artículo | - |
dc.identifier.doi | 10.1111/1574-6968.12098 | - |
dc.relation.publisherversion | http://dx.doi.org/10.1111/1574-6968.12098 | - |
dc.date.updated | 2013-07-11T13:08:23Z | - |
dc.description.version | Peer Reviewed | - |
dc.contributor.funder | Ministerio de Educación y Ciencia (España) | - |
dc.contributor.funder | Fundación Ramón Areces | - |
dc.relation.csic | Sí | - |
dc.identifier.funder | http://dx.doi.org/10.13039/100008054 | es_ES |
dc.identifier.pmid | 23398561 | - |
dc.type.coar | http://purl.org/coar/resource_type/c_6501 | es_ES |
item.openairetype | artículo | - |
item.grantfulltext | open | - |
item.cerifentitytype | Publications | - |
item.openairecristype | http://purl.org/coar/resource_type/c_18cf | - |
item.fulltext | With Fulltext | - |
item.languageiso639-1 | en | - |
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double polyphosphate.pdf | 1,42 MB | Adobe PDF | Visualizar/Abrir |
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