English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/79414
Share/Impact:
Statistics
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:

Title

The c-erbAα protooncogene induces apoptosis in glial cells via a protein kinase C- and bcl-2-suppressible mechanism

AuthorsLlanos, Susana; Caelles, Carme; Boscá, Lisardo ; Muñoz Terol, Alberto
Issue Date1998
PublisherWiley-Blackwell
CitationJournal of Neurochemistry 70(6): 2315-2326 (1998)
AbstractThe c-erbA protooncogene encodes the thyroid hormone (3,5,3'- triiodothyronine; T3) receptor α1 (TRα1). c-erbA/TRα1 is expressed in many cell types including glial cells, particularly in the immature state. We show here by morphological and biochemical criteria that c-erbA induces apoptosis of glial B3.1 cells in serum-deprived conditions. This effect is mostly T3 independent. Growth factors such as platelet-derived growth factor, basic fibroblast growth factor, or transforming growth factor-α prevent B3.1 + TRα1 cell death. Protein kinase C (PKC) activators also prevent the apoptosis phenomenon, an effect that was blocked by the PKC- specific inhibitor GF109203X. Expression of an exogenous bcl-2 gene led also to B3.1 + TRα1 cell survival. Neither a series of inhibitors including GF109203X nor T3 inhibits bcl-2 action, indicating that bcl-2 blocks a downstream step in the death-promoting process. B3.1 + TRα1 cell apoptosis is not blocked by caspase-1 or poly-ADP-ribosyltransferase inhibitors, suggesting that the activation of these classic pathways is not involved in the apoptotic mechanism. In addition, direct interaction with specific neuronal cells but not incubation with their conditioned medium inhibits also apoptosis of B3.1 + TRα1 cells. Our results show that c-erbA promotes an apoptotic process in glial B3.1 cells that is suppressible by PKC activation and bcl-2, probably by distinct mechanisms.
URIhttp://hdl.handle.net/10261/79414
DOI10.1046/j.1471-4159.1998.70062315.x
Identifiersdoi: 10.1046/j.1471-4159.1998.70062315.x
issn: 0022-3042
e-issn: 1471-4159
Appears in Collections:(IIBM) Artículos
Files in This Item:
File Description SizeFormat 
accesoRestringido.pdf15,38 kBAdobe PDFThumbnail
View/Open
Show full item record
Review this work
 

Related articles:


WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.