Por favor, use este identificador para citar o enlazar a este item:
http://hdl.handle.net/10261/79399
COMPARTIR / EXPORTAR:
SHARE CORE BASE | |
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE | |
Título: | Activation of the prolactin gene by peroxisome proliferator-activated receptor-α appears to be DNA binding-independent |
Autor: | Tolón, Rosa M. CSIC; Castillo, Ana I. CSIC ORCID; Aranda, Ana CSIC ORCID | Fecha de publicación: | 1998 | Editor: | American Society for Biochemistry and Molecular Biology | Citación: | Journal of Biological Chemistry 273(41): 26652-26661 (1998) | Resumen: | Although the effects of the peroxisome proliferator-activated receptors (PPARs) have been studied primarily in adipocytes and liver, the wide distribution of these receptors suggests that they might also play a role in other cell types. We present evidence that PPAR activators stimulate the expression of the prolactin gene in pituitary GH4C1 cells. Transfection assays in non-pituitary HeLa cells showed that stimulation of the prolactin promoter by PPARα requires the presence of the transcription factor GHF-1 (or Pit-1). Proximal promoter sequences confer responsiveness to PPARα, and activation by this receptor is lost concomitantly with the response to GHF- 1. Surprisingly, expression of the retinoid X receptor (RXR) abolishes stimulation by PPARα. Furthermore, the promoter region that confers PPARα responsiveness does not contain a PPAR response element. This suggests that the transcriptional effect of PPARα might be mediated by protein-protein interactions rather than by binding of PP RXR to the promoter. A direct interaction between PPARα and GHF-1 was confirmed by in vitro binding studies. Expression of the coactivators SRC-1 and CREB-binding protein, which bind to PPAR, also enhanced the responsiveness of the prolactin promoter to PPARα. Furthermore, CREB-binding protein also significantly increased activation by GHF-1, and both proteins associated in vitro. Thus, PPARα, a receptor that normally acts as a ligand-dependent transcription factor by binding to specific DNA sequences in one context, can also stimulate the prolactin promoter by association with GHF-1 and coactivator proteins. | URI: | http://hdl.handle.net/10261/79399 | DOI: | 10.1074/jbc.273.41.26652 | Identificadores: | doi: 10.1074/jbc.273.41.26652 issn: 0021-9258 e-issn: 1083-351X |
Aparece en las colecciones: | (IIBM) Artículos |
Ficheros en este ítem:
Fichero | Descripción | Tamaño | Formato | |
---|---|---|---|---|
accesoRestringido.pdf | 15,38 kB | Adobe PDF | Visualizar/Abrir |
CORE Recommender
SCOPUSTM
Citations
53
checked on 20-abr-2024
WEB OF SCIENCETM
Citations
50
checked on 24-feb-2024
Page view(s)
266
checked on 24-abr-2024
Download(s)
35
checked on 24-abr-2024
Google ScholarTM
Check
Altmetric
Altmetric
NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.