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Title

Autoinhibitory regulation of TrwK, an essential VirB4 ATPase in type IV secretion systems

AuthorsPeña, Alejandro ; Ripoll-Rozada, Jorge ; Zunzunegui, Sandra ; Cabezón, Elena ; Cruz, Fernando de la ; Arechaga, Ignacio
Issue Date2011
PublisherAmerican Society for Biochemistry and Molecular Biology
CitationJournal of Biological Chemistry 286(19): 17376-17382 (2011)
AbstractType IV secretion systems (T4SS) mediate the transfer of DNA and protein substrates to target cells. TrwK, encoded by the conjugative plasmid R388, is a member of the VirB4 family, comprising the largest and most conserved proteins of T4SS. In a previous work we demonstrated that TrwK is able to hydrolyze ATP. Here, based on the structural homology of VirB4 proteins with the DNA-pumping ATPase TrwB coupling protein, we generated a series of variants of TrwK where fragments of the C-terminal domain were sequentially truncated. Surprisingly, the in vitro ATPase activity of these TrwK variants was much higher than that of the wild-type enzyme. Moreover, addition of a synthetic peptide containing the amino acid residues comprising this C-terminal region resulted in the specific inhibition of the TrwK variants lacking such domain. These results indicate that the C-terminal end of TrwK plays an important regulatory role in the functioning of the T4SS.
URIhttp://hdl.handle.net/10261/78728
DOI10.1074/jbc.M110.208942
Identifiersdoi: 10.1074/jbc.M110.208942
issn: 0021-9258
e-issn: 1083-351X
Appears in Collections:(IBBTEC) Artículos
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