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Cloning of a cDNA encoding an Artemia franciscana Na/K ATPase α-subunit

AuthorsMacías, María-Teresa ; Palmero, Ignacio ; Sastre, Leandro
Issue Date1991
CitationGene 105(2): 197-204 (1991)
AbstractClones of cDNA that code for an isoform of the Artemia franciscana Na/K ATPase α subunit (NaKAα) have been isolated. The sequence of the longest of these clones (pArATNa136) is 3595 nucleotides; it codes for a 1004-amino acid protein whose sequence is identical to that of two previously sequenced artemia NaKAα peptides. The encoded protein is over 73% identical to Drosophila melanogaster and vertebrate NaKAαs, and 73.8% identical to another Artemia NaKAα isoform previously described (named α2850 in this article). The two Artemia cDNA clones code for mRNAs of different size; the clone pArATNa136 codes for a 4.5-kb mRNA while the α2850 clone codes for a 3.6-kb mRNA. The degree of homology and the different size of the mRNAs encoded by both cDNAs suggest that they code for two different isoforms of the protein.
Identifiersdoi: 10.1016/0378-1119(91)90151-Z
issn: 0378-1119
e-issn: 1879-0038
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