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dc.contributor.authorMeijer, Wilfried J. J.-
dc.contributor.authorSalas, Margarita-
dc.identifier.citationNucleic Acids Research, 2004, Vol. 32, No. 3 1166-1176en_US
dc.description.abstractVarious Escherichia coli promoters contain, in addition to the classical –35 and –10 hexamers, a third recognition element, named the UP element. Located upstream of the –35 box, UP elements stimulate promoter activity by forming a docking site for the C-terminal domain of the RNA polymerase subunit (CTD). Accumulating genetic, biochemical and structural information has provided a detailed picture on the molecular mechanism underlying UP element-dependent promoter stimulation in E.coli. However, far less is known about functional UP elements of Bacillus subtilis promoters. Here we analyse the strong early A-RNA polymerase-dependent promoters C2, A2c and A2b of the lytic B.subtilis phage phi 29. We demonstrate that the phage promoters contain functional UP elements although their contribution to promoter strength is very different. Moreover, we show that the UP element of the A2b promoter, being critical for its activity, is located further upstream of the –35 box than most E.coli UP elements. The importance of the UP elements for the phage promoters and how they relate to other UP elements are discussed.en_US
dc.description.sponsorshipThis investigation was supported by grants 2RO1 GM27242-24 from the National Institutes of Health, BMC2002-03818 from the Spanish Ministry of Science and Technology, and an Institutional grant from Fundación Ramón Areces to the Centro Biología Molecular ‘Severo Ochoa’. The ‘Ramon y Cajal’ programme of the Spanish ministry of Science and Technology supported W.J.J.M.en_US
dc.format.extent261594 bytes-
dc.publisherOxford University Pressen_US
dc.subjectBacillus subtilisen_US
dc.subjectPhage phi 29en_US
dc.titleRelevance of UP elements for three strong Bacillus subtilis phage phi 29 promotersen_US
dc.description.peerreviewedPeer revieweden_US
dc.contributor.funderNational Institutes of Health (US)-
dc.contributor.funderMinisterio de Ciencia y Tecnología (España)-
dc.contributor.funderFundación Ramón Areces-
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