Por favor, use este identificador para citar o enlazar a este item:
http://hdl.handle.net/10261/77781
COMPARTIR / EXPORTAR:
SHARE CORE BASE | |
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE | |
Título: | Protoporphyrin IX-dependent photodynamic production of endogenous ROS stimulates cell proliferation |
Autor: | Blázquez-Castro, Alfonso; Carrasco, Elisa CSIC ORCID; Calvo, María I.; Jaén, Pedro; Stockert, Juan Carlos CSIC; Juarranz, Ángeles; Sanz-Rodríguez, Francisco CSIC ORCID; Espada, Jesús CSIC ORCID | Fecha de publicación: | 2012 | Editor: | Elsevier | Citación: | European Journal of Cell Biology 91(3): 216-223 (2012) | Resumen: | Photodynamic therapy using methyl 5-aminolevulinate (MAL) as a precursor of the photosensitizing agent protoporphyrin IX is widely used in clinical practice for the treatment of different pathologies, including cancer. In this therapeutic modality, MAL treatment promotes the forced accumulation of the endogenous photoactive compound protoporphyrin IX in target malignant cells. Subsequent irradiation of treated tissues with an appropriate visible light source induces the production of reactive oxygen species (ROS) that, once accumulated above a critical level, promote cell death. Here we demonstrate that a photodynamic treatment with low MAL concentrations can be used to promote a moderate production of endogenous ROS, which efficiently stimulates cell growth in human immortalized keratinocytes (HaCaT). We also show that this proliferative response requires Src kinase activity and is associated to a transient induction of cyclin D1 expression. Taken together, these results demonstrate for the first time that a combination of light and a photoactive compound can be used to modulate cell cycle progression through Src kinase activation and that a moderate intracellular increase of photogenerated ROS efficiently stimulates cell proliferation. © 2011 Elsevier GmbH. | Versión del editor: | http://dx.doi.org/10.1016/j.ejcb.2011.12.001 | URI: | http://hdl.handle.net/10261/77781 | DOI: | 10.1016/j.ejcb.2011.12.001 | Identificadores: | doi: 10.1016/j.ejcb.2011.12.001 issn: 0171-9335 e-issn: 1618-1298 |
Aparece en las colecciones: | (IIBM) Artículos |
Ficheros en este ítem:
Fichero | Descripción | Tamaño | Formato | |
---|---|---|---|---|
Protoporphyrin IX.pdf | 1,19 MB | Adobe PDF | Visualizar/Abrir |
CORE Recommender
SCOPUSTM
Citations
53
checked on 16-abr-2024
WEB OF SCIENCETM
Citations
53
checked on 23-feb-2024
Page view(s)
380
checked on 23-abr-2024
Download(s)
543
checked on 23-abr-2024
Google ScholarTM
Check
Altmetric
Altmetric
NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.