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Título: | Serotonin 4 receptor (5-HT4R) internalization is isoform-specific: Effects of 5-HT and RS67333 on isoforms A and B |
Autor: | Mnie-Filali, Ouissame; Amrael, Mohammad Gholi; Benmbarek, S.; Archer-Lahlou, Élodie; Peñas-Cazorla, Raúl CSIC; Vilaró, Maria Teresa CSIC ORCID; Boye, Sandra M.; Piñeyro, Graciela | Palabras clave: | 5-HT4R isoforms Antidepressants Internalization Recycling RS67333 Trafficking |
Fecha de publicación: | 2010 | Editor: | Elsevier | Citación: | Cellular Signalling 22(3): 501-509 (2010) | Resumen: | Serotonin 4 receptors (5-HT4Rs) are particularly abundant within the limbic system, where they constitute potential targets for the development of novel, rapid acting antidepressants. However, the population of limbic 5-HT4Rs is not homogenous, comprising various isoforms of which 5-HT4(a) and 5-HT4(b) are among the most abundant variants. Sequence divergence at their C-termini is predictive of specificity in isoform signalling and regulation, but the differences, if any, remain ill-defined. The present study compared isoforms 5-HT4(a) and 5-HT4(b) in their ability to undergo endocytic regulation following exposure to 5-HT and to the putatively fast acting antidepressant RS67333. Both ligands differed in their ability to induce internalization of either isoform, 5-HT being more effective than RS67333 in HEK293 cells and in neurons. In contrast, trafficking induced by 5-HT was isoform-specific. In particular, while PKC, GRK2 and βarrestin were necessary for 5-HT4(a)R internalization, sequestration of 5-HT4(b)Rs required PKC but not GRK2 and relied significantly less on βarrestin. After endocytosis, isoform (b) appeared scattered throughout the intracellular compartment and efficiently recycled to the membrane upon agonist removal. Isoform (a) accumulated in the perinuclear compartment and displayed little recycling. Isoform-specific subcellular distribution was present in HEK293 cells and in neurons. In neurons, where internalization by RS67333 was more pronounced than in HEK293 cells, receptors internalized by this ligand followed the same distribution pattern as observed with 5-HT. These results point to isoform-related differences in the way that 5-HTRs respond to different ligands. Such diversity should be taken into account when developing therapeutic agents that target 5-HT4Rs. © 2009 Elsevier Inc. All rights reserved. | Versión del editor: | http://dx.doi.org/10.1016/j.cellsig.2009.11.004 | URI: | http://hdl.handle.net/10261/77669 | DOI: | 10.1016/j.cellsig.2009.11.004 | Identificadores: | doi: 10.1016/j.cellsig.2009.11.004 issn: 0898-6568 |
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