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Título: | A method to compare MALDI-TOF MS PMF spectra and its application in phyloproteomics |
Autor: | Ortea, Ignacio CSIC ORCID; Barros, Lorena CSIC; Cañas, Benito; Calo-Mata, Pilar CSIC; Barros-Velázquez, Jorge CSIC ORCID; Gallardo, José Manuel CSIC | Palabras clave: | Phyloproteomics MALDI-TOF MS PMF Shrimps |
Fecha de publicación: | 2009 | Editor: | Springer Nature | Citación: | Distributed Computing, Artificial Intelligence, Bioinformatics, Soft Computing, and Ambient Assisted Living: 1147-1153 (2009) | Serie: | Lecture Notes in Computer Science 5518 |
Resumen: | The suitability of a proteomic approach was explored to establish phylogenetic relationships among closely-related species. Decapoda penaeid shrimps were chosen as case study because these species have been widely studied and their phylogenetic relationships have been inferred by extensively validated methods, among which mitochondrial DNA-based PCR studies have provided relevant information Thus, MALDI-TOF mass spectrometry (MS) peptide mass fingerprinting (PMF) of arginine kinase was performed, this enzyme being selected from the sarcoplasmic proteome of such species due to the interspecific variability of their pI values. The presence or absence of selected peptides in the MS spectra was used as a molecular marker for phylogenetic analysis. Based on the cluster analysis of the MALDI-TOF PMF spectra obtained, a dendrogram was generated which could be validated with those obtained using DNA-based methods. | Descripción: | 7 pages.-- 10th International Work-Conference on Artificial Neural Networks, IWANN 2009 Workshops, Salamanca, Spain, June 10-12, 2009. Proceedings, Part II | Versión del editor: | http://dx.doi.org/10.1007/978-3-642-02481-8_174 | URI: | http://hdl.handle.net/10261/77367 | DOI: | 10.1007/978-3-642-02481-8_174 | ISBN: | 978-3-642-02480-1 |
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