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A standardized kinetic model to evaluate the antioxidant activity responses. The β-Carotene method as a case study.

AuthorsPrieto, Miguel Ángel ; Rodríguez-Amado, I. ; Vázquez, José Antonio ; Anders, Yvonne; Murado García, Miguel Anxo
KeywordsAntioxidant activity
β-carotene method
Mathematical modelling
Issue Date2012
PublisherSociedade Portuguesa de Química
CitationQualidade dos alimentos: novos desafios
AbstractThe β-carotene (βC) bleaching assay, a common method for evaluating antioxidant activity (AA), which operates on a system of lipid micelles in an aqueous environment is a well accepted model for testing the AA of foods. In this work, we present a highly reproducible procedure for microplate assay using a kinetic model for quantifying the AA, which produces stable and meaningful characterizations. The microplate assay provides an appropriate tool for ensuring that sample series with a large number of items can be simultaneously assessed. The application of these resources produce very consistent results, which provide robust and meaningful criteria to compare in detail the characteristics of antioxidant (A) agents.
Description4 páginas, 2 figuras.-- 11º Encontro de Química dos Alimentos, septiembre 2012
Appears in Collections:(IIM) Comunicaciones congresos
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