Por favor, use este identificador para citar o enlazar a este item:
http://hdl.handle.net/10261/76807
COMPARTIR / EXPORTAR:
SHARE CORE BASE | |
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE | |
Título: | Early thyroid hormone-induced gene expression changes in N2a-β neuroblastoma cells |
Autor: | Bedó, Gabriela; Pascual, Ángel CSIC; Aranda, Ana CSIC ORCID | Fecha de publicación: | 2011 | Editor: | Springer Nature | Citación: | Journal of Molecular Neuroscience 45(2): 76-86 (2011) | Resumen: | Thyroid hormone has long been known to regulate neural development. Hypothyroidism during pregnancy and early postnatal period has severe neurological consequences including even mental retardation. The purpose of this study was to characterize gene expression pattern during thyroid hormone-induced differentiation of neuro-2a β cells in order to select >direct response genes> for further analysis. In this neuroblastoma cell line, thyroid hormone blocks proliferation and induces differentiation. Changes in gene expression level were examined after a T3 treatment of 3 and 24 h using cDNA arrays. Sixteen genes were significantly up-regulated and 79 down-regulated by T3 treatment. Five up-regulated genes not previously described as regulated by thyroid hormone and selected for their putative significance to understand T3 action on cell differentiation, were verified by RT-PCR analysis. The transcription factors Phox2a and basic helix-loop-helix domain containing, class B2 mRNAs exhibited a clear increase after 3- and 24-h treatment. The guanine-nucleotide exchange factor RalGDS was greatly up-regulated after 3-h treatment but not 24 h after. The results suggest an early involvement of these genes in T3 action during neuroblastoma cell differentiation probably mediating later changes in gene expression pattern. © 2010 Springer Science+Business Media, LLC. | URI: | http://hdl.handle.net/10261/76807 | DOI: | 10.1007/s12031-010-9389-y | Identificadores: | doi: 10.1007/s12031-010-9389-y issn: 0895-8696 e-issn: 1559-1166 |
Aparece en las colecciones: | (IIBM) Artículos |
Ficheros en este ítem:
Fichero | Descripción | Tamaño | Formato | |
---|---|---|---|---|
accesoRestringido.pdf | 15,38 kB | Adobe PDF | Visualizar/Abrir |
CORE Recommender
SCOPUSTM
Citations
6
checked on 09-abr-2024
WEB OF SCIENCETM
Citations
5
checked on 24-feb-2024
Page view(s)
279
checked on 16-abr-2024
Download(s)
94
checked on 16-abr-2024
Google ScholarTM
Check
Altmetric
Altmetric
NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.