English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/76804
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:


Early otic development depends on autophagy for apoptotic cell clearance and neural differentiation

AuthorsRodríguez Aburto, María ; Sánchez-Calderón, Hortensia; Hurlé, Juan M.; Varela-Nieto, Isabel ; Magariños, Marta
Issue Date2012
PublisherNature Publishing Group
CitationCell Death and Disease 3: e394 (2012)
AbstractAutophagy is a highly regulated program of self-degradation of the cytosolic constituents that has key roles during early development and in adult cell growth and homeostasis. To investigate the role of autophagy in otic neurogenesis, we studied the expression of autophagy genes in early stages of chicken (Gallus gallus) inner ear development and the consequences of inhibiting the autophagic pathway in organotypic cultures of explanted chicken otic vesicles (OVs). Here we show the expression of autophagy-related genes (Atg) Beclin-1 (Atg6), Atg5 and LC3B (Atg8) in the otocyst and the presence of autophagic vesicles by using transmission electron microscopy in the otic neurogenic zone. The inhibition of the transcription of LC3B by using antisense morpholinos and of class III phosphatidylinositol 3-kinase with 3-methyladenine causes an aberrant morphology of the OV with accumulation of apoptotic cells. Moreover, inhibition of autophagy provokes the misregulation of the cell cycle in the otic epithelium, impaired neurogenesis and poor axonal outgrowth. Finally, our results indicate that autophagy provides the energy required for the clearing of neuroepithelial dying cells and suggest that it is required for the migration of otic neuronal precursors. Taken together, our results show for the first time that autophagy is an active and essential process during early inner ear development. © 2012 Macmillan Publishers Limited. All rights reserved.
Publisher version (URL)http://dx.doi.org/10.1038/cddis.2012.132
Identifiersdoi: 10.1038/cddis.2012.132
issn: 2041-4889
Appears in Collections:(IIBM) Artículos
Files in This Item:
File Description SizeFormat 
Early otic.pdf4,89 MBAdobe PDFThumbnail
Show full item record
Review this work

Related articles:

WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.