Por favor, use este identificador para citar o enlazar a este item:
http://hdl.handle.net/10261/76778
COMPARTIR / EXPORTAR:
SHARE BASE | |
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE | |
Título: | Down-regulation of the epidermal growth factor receptor by altering N-glycosylation: Emerging role of ß1,4-galactosyltransferases |
Autor: | Gabius, Hans-Joachim; Wouwer, Marlies van de CSIC; André, Sabine; Villalobo, Antonio CSIC ORCID | Fecha de publicación: | 2012 | Editor: | International Institute of Anticancer Research | Citación: | Anticancer Research 32(5): 1565-1572 (2012) | Resumen: | [Background/Aim]: Blocking N-glycosylation of the epidermal growth factor receptor (EGFR) by tunicamycin inhibits its cellular accumulation. Due to the toxic potential of this drug, finding less drastic routes to reduce EGFR expression is desirable. [Materials and Methods]: Four glycosylation mutants of Chinese hamster ovary (CHO) cells with defects in N-glycan processing and branch-end maturation were tested for EGFR gene expression, production, functionality and routing after transfection with a vector encoding for human EGFR. [Results]: Lack of conversion of paucimannosidic to hybrid/complex-type N-glycans and drastic reductions in sialylation/galactosylation did not lead to major effects. In contrast, EGFR expression in a mutant with reduced presence of ß1,4-galactosyltransferases-I-VI was markedly reduced. Misrouting or defects in transfection/transcription were excluded. [Conclusion]: ß1,4-Galactosyl-transferases warrant for further attention as effector(s) in order to attenuate EGFR-dependent signaling. | Versión del editor: | http://ar.iiarjournals.org/content/32/5/1565.abstract#corresp-1 | URI: | http://hdl.handle.net/10261/76778 | Identificadores: | issn: 0250-7005 e-issn: 1791-7530 |
Aparece en las colecciones: | (IIBM) Artículos |
Ficheros en este ítem:
Fichero | Descripción | Tamaño | Formato | |
---|---|---|---|---|
accesoRestringido.pdf | 15,38 kB | Adobe PDF | Visualizar/Abrir |
CORE Recommender
Page view(s)
298
checked on 22-abr-2024
Download(s)
52
checked on 22-abr-2024
Google ScholarTM
Check
NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.