Por favor, use este identificador para citar o enlazar a este item:
http://hdl.handle.net/10261/76761
COMPARTIR / EXPORTAR:
SHARE CORE BASE | |
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE | |
Título: | Effects of polyethylene and α-alumina particles on IL-6 expression and secretion in primary cultures of human osteoblastic cells |
Autor: | Rodrigo, A. M.; Martínez, M. E.; Saldaña, L.; Vallés, G.; Martínez, P.; González-Carrasco, José Luis CSIC ORCID CVN ; Cordero, J.; Munuera, L. | Fecha de publicación: | 2002 | Editor: | Elsevier | Citación: | Biomaterials 23: 901-908 (2002) | Resumen: | The effect of two biomaterials, polyethylene and α-alumina, on interleukin-6 (IL-6) secretion and expression has been studied in human osteoblasts in primary culture. Human osteoblastic cells were derived from fresh trabecular bone explants removed during total knee arthroplasty. On reaching confluence, cells were subcultured in 6 well plates; the resulting subcultures were incubated until confluence and polyethylene or α-alumina particles were added to some while the rest were left as controls. The IL-6 mRNA levels were assessed by reverse transcription (RT) followed by polymerase chain reaction (PCR). IL-6 secretion was measured in the conditioned medium. The IL-6 expression was higher in the presence of both biomaterials. Maximum expression occurred in response to a dose of 50mg particles/well with both biomaterials and was greater after polyethylene particle addition than after α-alumina particle addition at this dose. The maximum IL-6 secretion elicited by α-alumina was produced at 10mg particles/well while maximum response with polyethylene required 50mg/well. At a dose of 10mg/well, α-alumina particles induced more secretion than 10mg of polyethylene particles. Nevertheless, at a dose of 50mg/well maximum secretion was produced with polyethylene particles. In conclusion and in our experimental conditions, polyethylene as well as α-alumina increased both the expression and the secretion of IL-6 in human osteoblastic cells in primary culture and stimulation from polyethylene appears stronger than that from α-alumina at the same dose. © 2001 Elsevier Science Ltd. All rights reserved. | URI: | http://hdl.handle.net/10261/76761 | DOI: | 10.1016/S0142-9612(01)00200-9 | Identificadores: | doi: 10.1016/S0142-9612(01)00200-9 issn: 0142-9612 |
Aparece en las colecciones: | (CENIM) Artículos |
Ficheros en este ítem:
Fichero | Descripción | Tamaño | Formato | |
---|---|---|---|---|
accesoRestringido.pdf | 15,38 kB | Adobe PDF | Visualizar/Abrir |
CORE Recommender
SCOPUSTM
Citations
20
checked on 29-mar-2024
WEB OF SCIENCETM
Citations
16
checked on 24-feb-2024
Page view(s)
298
checked on 29-mar-2024
Download(s)
128
checked on 29-mar-2024
Google ScholarTM
Check
Altmetric
Altmetric
NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.