English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/75395
Share/Impact:
Statistics
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:
Title

Overexpression of ADC2 in Arabidopsis induces dwarfism and late-flowering through GA deficiency

AuthorsAlcázar, R.; García-Martínez, José-Luis ; Cuevas, J. C.; Tiburcio, A. F.; Altabella, Teresa
KeywordsOat arginine decarboxylase
Tobacco tissue-cultures
Polyamine biosynthesis
Gibberellin biosynthesis
Transgenic rice
Ornithine decarboxylase
Putrescine accumulation
Osmotic-stress
Oryza-sativa
Expression
Issue DateAug-2005
PublisherBlackwell Publishing
CitationPlant Journal 43/3: 425-436 (2005)
AbstractWe have obtained Arabidopsis thaliana transgenic plants constitutively overexpressing ADC2, one of the two genes encoding arginine decarboxylase (ADC) in Arabidopsis. These plants contained very high levels of putrescine (Put) but no changes were observed in spermidine and spermine contents. The results obtained from quantification of free and conjugated polyamines suggest that conjugation may be a limiting step for control of Put homeostasis within a non-toxic range for plant survival. Transgenic plants with increased levels of ADC2 transcript and elevated Put content showed dwarfism and late-flowering, and the phenotype was rescued by gibberellin A(3) (GA(3)) application. The contents of bioactive GA(4) and GA(1), and of GA(9) (a precursor of GA(4)), as well as the levels of AtGA20ox1, AtGA3ox1 and AtGA3ox3 transcripts (quantified by real-time PCR) were lower in the ADC2 overexpressor plants than in the wild type. No change in the expression of genes encoding earlier enzymes in the GA biosynthesis pathway was detected by microarray analysis. These results suggest that Put accumulation affects GA metabolism through the repression of biosynthetic steps catalyzed by GA 20-oxidase and GA 3-oxidase.
Publisher version (URL)http://dx.doi.org/10.1111/j.1365-313X.2005.02465.x
URIhttp://hdl.handle.net/10261/75395
DOI10.1111/j.1365-313X.2005.02465.x
ISSN0960-7412
Appears in Collections:(IBMCP) Artículos
Files in This Item:
File Description SizeFormat 
accesoRestringido.pdf15,38 kBAdobe PDFThumbnail
View/Open
Show full item record
Review this work
 

Related articles:


WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.