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Título: | Fluorescent protein vectors for promoter analysis in lactic acid bacteria and Escherichia coli |
Autor: | García-Cayuela, Tomás CSIC ORCID; Gómez de Cadiñanos, Luz P.; Mohedano Bonillo, Mari Luz CSIC ORCID ; Fernández de Palencia, P. CSIC; Boden, Daniel; Wells, Jerry; Peláez, Carmen CSIC ; López García, Paloma CSIC ORCID ; Requena, Teresa CSIC ORCID | Fecha de publicación: | 2012 | Editor: | Springer Nature | Citación: | Applied Microbiology and Biotechnology 96(1): 171-181 (2012) | Resumen: | Fluorescent reporter genes are valuable tools for real-time monitoring of gene expression in living cells. In this study we describe the construction of novel promoter-probe vectors containing a synthetic mCherry fluorescent protein gene, codon-optimized for lactic acid bacteria, divergently linked, or not, to a gene encoding the S65T and F64L variant of the green fluorescent protein. The utility of the transcriptional fusion vectors was demonstrated by the cloning of a single or two divergent promoter regions and by the quantitative evaluation of fluorescence during growth of Lactococcus lactis, Enterococcus faecalis, and Escherichia coli. | Descripción: | 29 p.- 4 tab.-2 fig. | Versión del editor: | http://dx.doi.org/10.1007/s00253-012-4087-z | URI: | http://hdl.handle.net/10261/74026 | DOI: | 10.1007/s00253-012-4087-z | ISSN: | 0175-7598 | E-ISSN: | 1432-0614 |
Aparece en las colecciones: | (CIB) Artículos (CIAL) Artículos |
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