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Título: | Development of an inducible system to control and easily monitor gene expression in Lactococcus lactis |
Autor: | Viegas, Sandra Cristina; Fernández de Palencia, P. CSIC; Amblar, Mónica CSIC ORCID; Arraiano, Cecilia María; López García, Paloma CSIC ORCID | Fecha de publicación: | 2004 | Editor: | Elsevier | Citación: | Plasmid 51(3): 256-264(2004) | Resumen: | This report describes the implementation and use of a maltose-inducible system for regulated gene expression in Lactococcus lactis. The system was established using Green Fluorescent Protein as reporter. The transcription of a gene of interest from the inducible promoter of pLS1RGFP plasmid vector can be easily monitored by fluorescence spectroscopy and microscopy. As an example, the lactococcal ribonuclease III was overproduced in an active form. © 2004 Elsevier Inc. All rights reserved. | Versión del editor: | http://dx.doi.org/10.1016/j.plasmid.2004.02.004 | URI: | http://hdl.handle.net/10261/73753 | DOI: | 10.1016/j.plasmid.2004.02.004 | ISSN: | 0147-619X | E-ISSN: | 1095-9890 |
Aparece en las colecciones: | (CIB) Artículos |
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