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Título : Changes induced by fe deficiency and fe resupply in the root protein profile of a peach-almond hybrid rootstock
Autor : Rodríguez-Celma, Jorge ; Lattanzio, Giuseppe ; Jiménez Tarodo, Sergio ; Briat, Jean François; Abadía Bayona, Javier ; Abadía Bayona, Anunciación ; Gogorcena Aoiz, Yolanda ; López-Millán, Ana Flor
Palabras clave : iron
two-dimensional gel electrophoresis
Fecha de publicación : mar-2013
Editor: American Chemical Society
Citación : Rodríguez-Celma J, Lattanzio G, Jiménez S, Briat JF, Abadía J, Abadía A, Gogorcena Y, López-Millán AF. Changes induced by fe deficiency and fe resupply in the root protein profile of a peach-almond hybrid rootstock. Journal of Proteome Research 12 (3): 1162-1172 (2013)
Resumen: The changes in the root extract protein profile of the Prunus hybrid GF 677 rootstock (P. dulcis × P. persica) grown in hydroponics as affected by Fe deficiency and short-term (24 h) Fe resupply have been studied by 2-dimensional gel electrophoresis-based techniques. A total of 335 spots were consistently found in the gels. Iron deficiency caused above 2-fold increases or >50% decreases in the relative abundance in 10 and 6 spots, respectively, whereas one spot was only detected in Fe-deficient plants. Iron resupply to Fe-deficient plants caused increases and decreases in relative abundance in 15 and 16 spots, respectively, and one more spot was only detected in Fe-resupplied Fe-deficient plants. Ninety-five percent of the proteins changing in relative abundance were identified using nanoliquid chromatography-tandem mass spectrometry. Defense responses against oxidative and general stress accounted for 50% of the changes in Fe-deficient roots. Also, a slight induction of the glycolysis-fermentation pathways was observed in GF 677 roots with Fe deficiency. The root protein profile of 24 h Fe-resupplied plants was similar to that of Fe-deficient plants, indicating that the deactivation of Fe-deficiency metabolic responses is slow. Taken together, our results suggest that the high tolerance of GF 677 rootstock to Fe deficiency may be related to its ability to elicit a sound defense response against both general and oxidative stress.
Descripción : 11 Pags.
Versión del editor: http://dx.doi.org/10.1021/pr300763c
URI : http://hdl.handle.net/10261/73567
DOI: 10.1021/pr300763c
ISSN: 1535-3893
E-ISSN: 1535-3907
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