English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/73566
Share/Impact:
Statistics
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:

Title

Endoglin expression regulates basal and TGF-β1-induced extracellular matrix synthesis in cultured L6E9 myoblasts

AuthorsObreo, Juana; Diez-Marques, Luisa M.; Lamas Peláez, Santiago ; Düwell, Annette; Eleno, Nélida; Bernabéu, Carmelo ; Pandiella, Atanasio ; López-Novoa, José M.; Rodríguez-Barbero, Alicia
Issue Date2004
PublisherS. Karger AG
CitationCellular Physiology and Biochemistry 14(4-6):301-310(2004)
AbstractBackground/aims: TGF-β1 plays a major role in extracellular matrix (ECM) accumulation in tissue fibrosis. Connective tissue growth factor appears to play a critical role in this effect. Endoglin is a component of the transforming growth factor b (TGF-β) receptor complex. Endoglin is upregulated by TGF-β1, but its functional role in ECM regulation is unknown. Using rat myoblasts as a model system, we have assessed the role of endoglin on regulating CTGF expression and ECM synthesis and accumulation in the presence or absence of TGF-β1. Methods: L6E9 myoblast cell line was transfected with human endoglin, and collagen, fibronectin and CTGF production was assessed by Western blot and by proline incorporation to collagen proteins. Results: Northern blot analysis revealed that parental rat myoblasts L6E9 do not express endogenous endoglin. Upon endoglin transfection, endoglin-expressing cells displayed a decreased CTGF expression and decreased collagen and fibronectin accumulation respect to mock transfectants. Northern blot analysis also revealed a decreased α 2 (I) procollagen mRNA expression in endoglin transfectants. TGF-β1 treatment induced an increase in CTGF expression and collagen synthesis and accumulation in L6E9 myoblasts. This effect was significantly lower in endoglin-transfected than in mock-transfected cells. Conclusion: These results demonstrate that endoglin expression negatively regulates basal and TGF-β1-induced CTGF and collagen expression and synthesis
Publisher version (URL)http:dx.doi.org/10.1159/000080340
URIhttp://hdl.handle.net/10261/73566
DOI10.1159/000080340
ISSN1015-8987
E-ISSN1421-9778
Appears in Collections:(CIB) Artículos
Files in This Item:
File Description SizeFormat 
restringido.pdf21,67 kBAdobe PDFThumbnail
View/Open
Show full item record
Review this work
 

Related articles:


WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.