English   español  
Por favor, use este identificador para citar o enlazar a este item: http://hdl.handle.net/10261/73429
Título

Spatially resolved analysis of small molecules by matrix-assisted laser desorption/ionization mass spectrometric imaging (MALDI-MSI)

AutorPeukert, Manuela; Matros, Andrea; Lattanzio, Giuseppe ; Kaspar, Stephanie; Abadía Bayona, Javier ; Mock, Hans-Peter
Palabras clavebarley grains
MALDI-MSI
sample preparation
small molecules
tobacco roots
Fecha de publicaciónfeb-2012
EditorJohn Wiley & Sons
CitaciónPeukert M, Matros A, Lattanzio G, Kaspar S, Abadía J, Mock HP. Spatially resolved analysis of small molecules by matrix-assisted laser desorption/ionization mass spectrometric imaging (MALDI-MSI). New Phytologist 193 (3): 806-815 (2012)
ResumenMatrix-assisted laser desorption/ionization mass spectrometric imaging (MALDI-MSI) of tissues provides the means to analyse the spatial distributions of small molecules and proteins within tissues. This imaging technique is commonplace in medicinal and pharmaceutical research, but its application in plant science is very recent. Broader introduction requires specific adaptations for plant tissues. Sample preparation is of paramount importance in order to obtain high-quality spectra providing sufficient spatial resolution for compounds. Optimization is required for sectioning, choice of matrix and means of matrix deposition. Here, we present our current protocols for the detection of small molecules in cryodissected immature barley (Hordeum vulgare) grains and tobacco (Nicotiana tabacum) roots. Examples of MALDI-MSI measurements are provided, and the level of reproducibility across biological replicates is addressed. Furthermore, our approaches for the validation of distribution patterns and for the identification of molecules are described. Finally, we discuss how MALDI-MSI can contribute to applied plant research.
Descripción10 Pags., 6 Figs., 1 Tabl.
Versión del editorhttp://dx.doi.org/10.1111/j.1469-8137.2011.03970.x
URIhttp://hdl.handle.net/10261/73429
DOI10.1111/j.1469-8137.2011.03970.x
ISSN0028-646X
E-ISSN1469-8137
Aparece en las colecciones: (EEAD) Artículos
Ficheros en este ítem:
Fichero Descripción Tamaño Formato  
mod vers.aut pend.pdf20,83 kBAdobe PDFVista previa
Visualizar/Abrir
Mostrar el registro completo
 

Artículos relacionados:


NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.