English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/73077
Share/Impact:
Statistics
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:

Title

Differences in the peripheral immune response between lambs and adult ewes experimentally infected with Mycobacterium avium subspecies paratuberculosis

AuthorsDelgado, L. ; Juste, R. A.; Muñoz, María; Morales, S.; Benavides, Julio ; Ferreras, Mª del Carmen ; García Marín, Juan Francisco ; Pérez Pérez, Valentín
KeywordsSheep
Paratuberculosis
Age
IFN-y
ELISA
Skin test
Issue Date2012
PublisherElsevier
CitationVeterinary Immunology and Immunopathology 145(1-2): 23-31
AbstractThe peripheral immune response, and its relationship with the outcome of the infection according to the age of the animal, has been investigated in young lambs and adult ewes experimentally infected with two different doses of Mycobacterium avium subspecies paratuberculosis (Map). Sixteen 1.5-month-old lambs out of 24 and 23 adult ewes out of 30 were orally challenged with an ovine Map field isolate. Animals were divided into two groups: HD, infected with a higher dose of Map and LD, with a lower dose. The remaining animals were used as uninfected control groups. Animals were euthanized at 110–120 and 210–220 days post-infection (dpi). Along the experiment, the humoral response and the specific and non-specific IFN-γ production were assessed. An intradermal skin test (IDT), using avian PPD, was also performed at 90 and 195 dpi. Samples of intestine and related lymphoid tissue were taken for histological, bacteriological and PCR studies. The Ab and IFN-γ production as well as the IDT response appeared earlier and with more intensity in the adult ewes compared to the lambs. The basal non-specific IFN-γ levels increased only in the adult ewes from the HD group. Animals from the LD and HD groups were positive to PCR; however, lesions consistent with paratuberculosis were exclusively observed in the HD group, both in lambs and in adult sheep, but they only progressed to more advanced stages in the former. These results suggest that the peripheral immune response induced by Map infection in the adult ewes is more efficient to control the progression of the infection than in lambs. This could likely be due to the existence of previous contacts with Map or other mycobacteria in the adult sheep compared to the young lambs.
Description9 páginas, 4 figuras.
Publisher version (URL)http://dx.doi.org/10.1016/j.vetimm.2011.10.005
URIhttp://hdl.handle.net/10261/73077
DOI10.1016/j.vetimm.2011.10.005
ISSN0165-2427
Appears in Collections:(IGM) Artículos
Files in This Item:
File Description SizeFormat 
Embargo. Digital.CSIC..pdf21,67 kBAdobe PDFThumbnail
View/Open
Show full item record
Review this work
 

Related articles:


WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.