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Neutralizing antibodies against rotavirus produced in transgenically labelled purple tomatoes

AuthorsJuárez, Paloma ; Presa, Silvia ; Espí, Joaquín; Pineda Chaza, Benito José ; Antón Martínez, María Teresa ; Moreno Ferrero, Vicente ; Buesa, Javier; Granell, Antonio ; Orzáez, Diego
KeywordsCarotenoid biosynthesis
Secretory antibodies
Fusion protein
Capsid protein
Issue DateApr-2012
CitationPlant Biotechnology Journal 10(3): 341-352 (2012)
AbstractEdible fruits are inexpensive biofactories for human health-promoting molecules that can be ingested as crude extracts or partially purified formulations. We show here the production of a model human antibody for passive protection against the enteric pathogen rotavirus in transgenically labelled tomato fruits. Transgenic tomato plants expressing a recombinant human immunoglobulin A (hIgA_2A1) selected against the VP8* peptide of rotavirus SA11 strain were obtained. The amount of hIgA_2A1 protein reached 3.6 +/- 0.8% of the total soluble protein in the fruit of the transformed plants. Minimally processed fruit-derived products suitable for oral intake showed anti-VP8* binding activity and strongly inhibited virus infection in an in vitro virus neutralization assay. In order to make tomatoes expressing hIgA_2A1 easily distinguishable from wild-type tomatoes, lines expressing hIgA_2A1 transgenes were sexually crossed with a transgenic tomato line expressing the genes encoding Antirrhinum majus Rosea1 and Delila transcription factors, which confer purple colour to the fruit. Consequently, transgenically labelled purple tomato fruits expressing hIgA_2A1 have been developed. The resulting purple-coloured extracts from these fruits contain high levels of recombinant anti-rotavirus neutralizing human IgA in combination with increased amounts of health-promoting anthocyanins.
DescriptionEl pdf del artículo es la versión pre-print.
Publisher version (URL)http://dx.doi.org/10.1111/j.1467-7652.2011.00666.x
Appears in Collections:(IBMCP) Artículos
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