English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/72876
Share/Impact:
Statistics
logo share SHARE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:

Title

Exploiting multisite gateway and pENFRUIT plasmid collection for fruit genetic engineering

AuthorsHueso Estornell, Leandro ; Granell, Antonio ; Orzáez, Diego
KeywordsAgrobacterium
Cloning
Issue Date2012
PublisherSpringer
CitationTransgenic Plants (Cap.28): 351-368 (2012)
Methods in Molecular Biology 847
AbstractMultiSite Gateway cloning techniques based on homologous recombination facilitate the combinatorial assembly of basic genetic pieces (i.e., promoters, CDS, and terminators) into gene expression or gene silencing cassettes. pENFRUIT is a collection of MultiSite Triple Gateway Entry vectors dedicated to genetic engineering in fruits. It comprises a number of fruit-operating promoters as well as C-terminal tags adapted to the Gateway standard. In this way, flanking regulatory/labeling sequences can be easily Gateway-assembled with a given gene of interest for its ectopic expression or silencing in fruits. The resulting gene constructs can be analyzed in stable transgenic plants or in transient expression assays, the latter allowing fast testing of the increasing number of combinations arising from MultiSite methodology. A detailed description of the use of MultiSite cloning methodology for the assembly of pENFRUIT elements is presented.
DescriptionEl pdf es la versión post-print.
Publisher version (URL)http://dx.doi.org/10.1007/978-1-61779-558-9_28
URIhttp://hdl.handle.net/10261/72876
DOI10.1007/978-1-61779-558-9_28
ISBN978-1-61779-557-2
Appears in Collections:(IBMCP) Libros y partes de libros
Files in This Item:
File Description SizeFormat 
Exploiting multisite.pdf883,99 kBAdobe PDFThumbnail
View/Open
Show full item record
Review this work
 

Related articles:


WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.