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http://hdl.handle.net/10261/72739
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Campo DC | Valor | Lengua/Idioma |
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dc.contributor.author | Sampedro, Nagore | - |
dc.contributor.author | Franco, José M. | - |
dc.contributor.author | Zapata, Manuel | - |
dc.contributor.author | Riobó, Pilar | - |
dc.contributor.author | Garcés, Esther | - |
dc.contributor.author | Penna, Antonella | - |
dc.contributor.author | Caillaud, Amandine | - |
dc.contributor.author | Diogène, Jorge | - |
dc.contributor.author | Cacho, Emiliano | - |
dc.contributor.author | Camp, Jordi | - |
dc.date.accessioned | 2013-03-22T10:42:29Z | - |
dc.date.available | 2013-03-22T10:42:29Z | - |
dc.date.issued | 2013 | - |
dc.identifier.citation | Harmful Algae 25: 26-38 (2013) | es_ES |
dc.identifier.issn | 1568-9883 | - |
dc.identifier.uri | http://hdl.handle.net/10261/72739 | - |
dc.description | 13 páginas, 8 tablas, 6 figuras | es_ES |
dc.description.abstract | The toxicity of Alexandrium andersonii Balech is unclear and its intraspecific variability has yet to be studied. To address these gaps in our knowledge, in the present work five strains of A. andersonii from four different localities were characterized. The results showed that despite genetic homogeneity in the 5.8-ITS (internal transcribed spacer) and large subunit (LSU) regions and similar growth rates, strains originating from different locations varied with respect to cell size, the ratios of certain pigments, and their growth patterns. Cultures of the strains grown at 20 °C were analyzed for toxicity using four different methodologies. The two officially established methods, mouse bioassay and high-performance liquid chromatography with fluorescence detection (HPLC-FLD) and post-column reaction analysis of PSP toxins, failed to show the toxicity of any strain. Strains grown at 14 °C were also negative for PSP toxins by HPLC-FLD. However, strains grown at 20 °C exhibited both a response characteristic of the presence of toxin-inhibiting voltage-gated sodium channels, as demonstrated in a neuroblastoma neuro-2a cell-based assay, as well as hemolytic activity in a sheep red blood cell assay. | es_ES |
dc.description.sponsorship | Financial support was provided by the Agència Catalana de l’Aigua (Department de Medi Ambient, Generalitat de Catalunya) and the CSIC through the contract “Pla de vigilància de fitoplàncton nociu i tòxic a la Costa Catalana;” by the “Xarxa de Referència de Recerca i Desenvolupament en Aqüicultura”; project JACUMAR/2007/SANIDAD ANIMAL/PSP2; and project PRIN 2009, financed by the Italian Ministry of Education, University and Research | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | Elsevier | es_ES |
dc.rights | openAccess | es_ES |
dc.subject | Alexandrium andersonii | es_ES |
dc.subject | Cytotoxicity assays | es_ES |
dc.subject | Intraspecific variability | es_ES |
dc.subject | Mediterranean Sea | es_ES |
dc.subject | PSP | es_ES |
dc.subject | Toxicity | es_ES |
dc.title | The toxicity and intraspecific variability of Alexandrium andersonii Balech | es_ES |
dc.type | artículo | es_ES |
dc.identifier.doi | 10.1016/j.hal.2013.02.003 | - |
dc.description.peerreviewed | Peer reviewed | es_ES |
dc.relation.publisherversion | http://dx.doi.org/10.1016/j.hal.2013.02.003 | es_ES |
dc.type.coar | http://purl.org/coar/resource_type/c_6501 | es_ES |
item.openairetype | artículo | - |
item.grantfulltext | open | - |
item.cerifentitytype | Publications | - |
item.openairecristype | http://purl.org/coar/resource_type/c_18cf | - |
item.fulltext | With Fulltext | - |
item.languageiso639-1 | en | - |
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HARALG-S-12-00259[2].pdf | 1,11 MB | Adobe PDF | Visualizar/Abrir |
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