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Title

The toxicity and intraspecific variability of Alexandrium andersonii Balech

AuthorsSampedro, Nagore ; Franco, José M. ; Zapata, Manuel ; Riobó, Pilar ; Garcés, Esther ; Penna, Antonella; Caillaud, Amandine; Diogène, Jorge; Cacho, E.; Camp, Jordi
KeywordsAlexandrium andersonii
Cytotoxicity assay
Intraspecific variability
Mediterranean Sea
PSP
Toxicity
Issue Date2013
PublisherElsevier
CitationHarmful Algae 25: 26-38 (2013)
AbstractThe toxicity of Alexandrium andersonii Balech is unclear and its intraspecific variability has yet to be studied. To address these gaps in our knowledge, in the present work five strains of A. andersonii from four different localities were characterized. The results showed that despite genetic homogeneity in the 5.8-ITS (internal transcribed spacer) and large subunit (LSU) regions and similar growth rates, strains originating from different locations varied with respect to cell size, the ratios of certain pigments, and their growth patterns. Cultures of the strains grown at 20 °C were analyzed for toxicity using four different methodologies. The two officially established methods, mouse bioassay and high-performance liquid chromatography with fluorescence detection (HPLC-FLD) and post-column reaction analysis of PSP toxins, failed to show the toxicity of any strain. Strains grown at 14 °C were also negative for PSP toxins by HPLC-FLD. However, strains grown at 20 °C exhibited both a response characteristic of the presence of toxin-inhibiting voltage-gated sodium channels, as demonstrated in a neuroblastoma neuro-2a cell-based assay, as well as hemolytic activity in a sheep red blood cell assay.
Description13 páginas, 8 tablas, 6 figuras
Publisher version (URL)http://dx.doi.org/10.1016/j.hal.2013.02.003
URIhttp://hdl.handle.net/10261/72739
DOI10.1016/j.hal.2013.02.003
ISSN1568-9883
Appears in Collections:(IIM) Artículos
(ICM) Artículos
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