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Título: | Regulation of gibberellin 20-oxidase gene expression and gibberellin content in citrus by temperature and citrus exocortis viroid |
Autor: | Vidal, Ana M.; Ben-Cheikh, W.; Talón, M.; García-Martínez, José-Luis CSIC ORCID | Palabras clave: | cDNA clones Biosynthesis Cloning Pea Arabidopsis Enzyme Apple Seeds |
Fecha de publicación: | jul-2003 | Editor: | Springer Nature | Citación: | Plant 217/3: 442-448 (2003) | Resumen: | A cDNA clone coding for a gibberellin (GA) 20-oxidase (CcGA20ox1), an enzyme of GA blosynthesis, which when expressed in vitro catalyzed the conversion of GA(12) to GA(9) and of GA(53) to GA(20), was isolated from the citrus hybrid Carrizo citrange (Citrus sinensis x Poncirus trifoliata). Transcripts of CeGA20ox1 were abundant in the apex and leaves and much less abundant in internodes, nodes and roots. Seedlings of Carrizo citrange cultured under a 32degreesC/27degreesC (day/ night) regime elongated more than seedlings growing under 17degreesC/12degreesC conditions. The effect of higher temperature was associated with more CcGA20ox1 transcripts and with higher content of GA(1), the main active GA in citrus, in the shoot. The infection of Etrog citron (Citrus medica) plants with citrus exocortis viroid (CEW), which produces a stunted phenotype, reduced the levels of transcripts in the apical shoot hybridizing to the gene CcGA20ox1 of Carrizo citrange and the content of GA(1). Thus GA(1) content correlated with CcGA20ox1 transcript levels. In contrast, results for gibberellic acid (GA(3)) and paclobutrazol applications to Carrizo citrange showed that CcGA20ox1 expression was subject to feed-back regulation. These observations indicate that the feed-back regulation of GA20ox operates mostly when the levels of active GAs have been dramatically altered. The results also show that the growth reduction induced by environmental (temperature) and biotic (CEVd) factors may be partially due to the modulation of the expression of GA20ox genes. | Versión del editor: | http://dx doi10.1007/s00425-003-0999-2 | URI: | http://hdl.handle.net/10261/72641 | ISSN: | 0032-0935 |
Aparece en las colecciones: | (IBMCP) Artículos |
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