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Título: | Mitochondrial [Ca2+] oscillations driven by local high [Ca 2+] domains generated by spontaneous electric activity |
Autor: | Villalobos, Carlos CSIC ORCID; Núñez, Lucía CSIC ORCID; Chamero, Pablo; Alonso, María Teresa; García-Sancho, Javier CSIC ORCID | Fecha de publicación: | sep-2001 | Editor: | American Society for Biochemistry and Molecular Biology | Citación: | Journal of Biological Chemistry 276(43): 40293-40297 (2001) | Resumen: | Mitochondria take up calcium during cell activation thus shaping Ca2+ signaling and exocytosis. In turn, Ca2+ uptake by mitochondria increases respiration and ATP synthesis. Targeted aequorins are excellent Ca2+ probes for subcellular analysis, but single-cell imaging has proven difficult. Here we combine virus-based expression of targeted aequorins with photon-counting imaging to resolve dynamics of the cytosolic, mitochondrial, and nuclear Ca2+ signals at the single-cell level in anterior pituitary cells. These cells exhibit spontaneous electric activity and cytosolic Ca2+oscillations that are responsible for basal secretion of pituitary hormones and are modulated by hypophysiotrophic factors. Aequorin reported spontaneous [Ca2+] oscillations in all the three compartments, bulk cytosol, nucleus, and mitochondria. Interestingly, a fraction of mitochondria underwent much larger [Ca2+] oscillations, which were driven by local high [Ca2+] domains generated by the spontaneous electric activity. These oscillations were large enough to stimulate respiration, providing the basis for local tune-up of mitochondrial function by the Ca2+ signal. | Descripción: | El pdf del artículo es el manuscrito de autor. | Versión del editor: | http://dx.doi.org/10.1074/jbc.C100465200 | URI: | http://hdl.handle.net/10261/71796 | DOI: | 10.1074/jbc.C100465200 | ISSN: | 0021-9258 | E-ISSN: | 1083-351X |
Aparece en las colecciones: | (IBGM) Artículos |
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Mitochondrial [Ca2+].pdf | 324,09 kB | Adobe PDF | Visualizar/Abrir |
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