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Molecular characterization of intrinsic and acquired antibiotic resistance in lactic acid bacteria and bifidobacteria

AuthorsAmmor, Mohammed Salim ; Flórez García, Ana Belén ; H. A. M. van Hoek, Angela; González de los Reyes-Gavilán, Clara ; J.M. Aarts, Henk; Margolles Barros, Abelardo ; Mayo Pérez, Baltasar
KeywordsBacterias ácido-lácticas
Resistencia a los antibioticos
Issue Date2008
PublisherS. Karger AG
CitationJournal of Molecular Microbiology and Biotechnonoly 14(1-3): 6-15 (2008)
AbstractThe minimum inhibitory concentrations (MICs) of 6 different antibiotics (chloramphenicol, clindamycin, erythromycin, streptomycin, tetracycline and vancomycin) were determined for 143 strains of lactic acid bacteria and bifidobacteria using the Etest. Different MICs were found for different species and strains. Based on the distribution of these MIC values, most of the strains were either susceptible or intrinsically resistant to these antibiotics. However, the MIC range of some of these antibiotics showed a bimodal distribution, which suggested that some of the tested strains possess acquired antibiotic resistance. Screening for resistance genes was performed by PCR using specific primers, or using a DNA microarray with around 300 nucleotide probes representing 7 classes of antibiotic resistance genes. The genes identified encoded resistance to tetracycline [tet(M), tet(W), tet(O) and tet(O/W)], erythromycin and clindamycin [erm(B)] and streptomycin [aph(E) and sat(3)]. Internal portions of some of these determinants were sequenced and found to be identical to genes described in other bacteria. All resistance determinants were located on the bacterial chromosome, except for tet(M), which was identified on plasmids in Lactococcus lactis. The contribution of intrinsic multidrug transporters to the antibiotic resistance was investigated by cloning and measuring the expression of Bifidobacterium breve genes in L. lactis.
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