English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/7098
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:


Two Different Tetracycline Resistance Mechanisms, Plasmid-Carried tet(L) and Chromosomally Located Transposon-Associated tet(M), Coexist in Lactobacillus sakei Rits 9

AuthorsAmmor, Mohammed Salim ; Gueimonde Fernández, Miguel ; Danielsen, Morten; Zagorec, Monique; H. A. M. van Hoek, Angela; González de los Reyes-Gavilán, Clara ; Mayo Pérez, Baltasar ; Margolles Barros, Abelardo
KeywordsLactobacillus sakei
Issue Date11-Jan-2008
PublisherAmerican Society for Microbiology
CitationApplied and Environmental Microbiology 74(5): 1394-1401 (2008)
AbstractLactobacillus sakei is extensively used as functional starter culture in fermented meat products. One of the safety criteria of a starter culture is the absence of potentially transferable antibiotic resistance determinants. However, tetracycline-resistant L. sakei strains have already been observed. In this paper, we show that tetracycline resistance in L. sakei Rits 9, a strain isolated from Italian Sola cheese made from raw milk, is mediated by a transposon-associated tet(M) gene coding for a ribosomal protection protein and a plasmid-carried tet(L) gene coding for a tetracycline efflux pump. pLS55, the 5-kb plasmid carrying the tet(L) gene, is highly similar to the pMA67 plasmid recently described for Paenibacillus larvae, a species pathogenic to honeybees. pLS55 could be transferred by electroporation into the laboratory strain L. sakei 23K. While the L. sakei 23K transformant containing pLS55 displayed an intermediate tetracycline resistance level (MIC, <32 µg/ml), L. sakei Rits 9, containing both tetracycline-resistant determinants, had a MIC of <256 µg/ml, suggesting that Tet L and Tet M confer different levels of resistance in L. sakei. Remarkably, in the absence of tetracycline, a basal expression of both genes was detected for L. sakei Rits 9. In addition, subinhibitory concentrations of tetracycline affected the expression patterns of tet(M) and tet(L) in different ways: the expression of tet(M) was induced only at high tetracycline concentrations, whereas the expression of tet(L) was up-regulated at lower concentrations. This is the first time that two different mechanisms conferring resistance to tetracycline are characterized for the same strain of a lactic acid bacterium
Appears in Collections:(IPLA) Artículos
Files in This Item:
File Description SizeFormat 
AEM-tetraciclina-Ammor-2008.pdf426,24 kBAdobe PDFThumbnail
Show full item record
Review this work

Related articles:

WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.