English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/7062
Title: Quantitative assessment of faecal bifidobacterial populations by real-time PCR using lanthanide probes
Authors: Gueimonde Fernández, Miguel; Debor, Linde; Tölkko, Sattu; Jokisalo, Erik; Salminen, Seppo
Keywords: Bifidobacterias
Microbiótica intestinal
PCR cuantitativa
Issue Date: 2007
Publisher: Society for Applied Microbiology
Citation: Journal of Applied Microbiology 102(4): 1116-1122 (2007)
Abstract: Aim: To develop real-time quantitative PCR methods, based on the use of probes labelled with a stable fluorescent lanthanide chelate, for the quantification of different human faecal bifidobacterial populations. Methods and Results: The designed quantitative PCR assays were found to be specific for the corresponding Bifidobacterium species or groups (Bifidobacterium longum group, Bifidobacterium catenulatum group, Bifidobacterium adolescentis, Bifidobacterium breve, Bifidobacterium angulatum, Bifidobacterium bifidum and Bifidobacterium dentium). The detection limits of the methodologies used ranged between 2 · 105 and 9 · 103 cells g)1 of faeces. The applicability of the developed assays was tested by analysing 20 human faecal samples. Bif. longum group was found to be the qualitatively and quantitatively predominant bifidobacterial group. Conclusions: The real-time PCR procedures developed here are specific, accurate, rapid and easy methods for the quantification of Bifidobacterium groups or species in human faecal samples. Significance and Impact of the Study: The developed procedures will facilitate rapid and objective counting of large numbers of samples increasing our knowledge on the role of gut bifidobacterial microbiota in health and disease. This will contribute to the efficient use of intestinal bacterial assays in research, food and pharmaceutical development as well as in the assessment of dietary management of diseases.
URI: http://hdl.handle.net/10261/7062
DOI: 10.1111/j.1365-2672.2006.03145.x
ISSN: 1364-5072
E-ISSN: 1365-2672
Appears in Collections:(IPLA) Artículos
Files in This Item:
File Description SizeFormat 
Gueimonde J Appl Microbiol 2007 102.pdf473,88 kBAdobe PDFThumbnail
Show full item record

WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.