Ciencia y Tecnología de Alimentos >
Instituto de Productos Lácteos de Asturias (IPLA) >
(IPLA) Artículos >
Open Access item Quantitative assessment of faecal bifidobacterial populations by real-time PCR using lanthanide probes
|Authors:||Gueimonde Fernández, Miguel|
|Keywords:||Bifidobacterias, Microbiótica intestinal, PCR cuantitativa|
|Publisher:||Society for Applied Microbiology|
|Citation:||Journal of Applied Microbiology 102 (4), 1116-1122 (2007)|
|Abstract:||Aim: To develop real-time quantitative PCR methods, based on the use of
probes labelled with a stable fluorescent lanthanide chelate, for the quantification
of different human faecal bifidobacterial populations.
Methods and Results: The designed quantitative PCR assays were found to
be specific for the corresponding Bifidobacterium species or groups (Bifidobacterium
longum group, Bifidobacterium catenulatum group, Bifidobacterium
adolescentis, Bifidobacterium breve, Bifidobacterium angulatum, Bifidobacterium
bifidum and Bifidobacterium dentium). The detection limits of the methodologies
used ranged between 2 · 105 and 9 · 103 cells g)1 of faeces. The applicability
of the developed assays was tested by analysing 20 human faecal samples.
Bif. longum group was found to be the qualitatively and quantitatively predominant
Conclusions: The real-time PCR procedures developed here are specific, accurate,
rapid and easy methods for the quantification of Bifidobacterium groups
or species in human faecal samples.
Significance and Impact of the Study: The developed procedures will facilitate
rapid and objective counting of large numbers of samples increasing our knowledge
on the role of gut bifidobacterial microbiota in health and disease. This
will contribute to the efficient use of intestinal bacterial assays in research, food
and pharmaceutical development as well as in the assessment of dietary management
|Appears in Collections:||(IPLA) Artículos|
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.