English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/7048
Title: Adaptation and Response of Bifidobacterium animalis subsp. lactis to Bile: a Proteomic and Physiological Approach
Authors: Sánchez García, Borja; Champomier-Vergès, Marie-Christine; Stuer-Lauridsen, Birgitte; Ruas-Madiedo, Patricia; Anglade, Patricia; Baraige, Fabienne; González de los Reyes-Gavilán, Clara; Johansen, Eric; Zagorec, Monique; Margolles Barros, Abelardo
Keywords: Bifidobacterias
Issue Date: 7-Sep-2007
Publisher: American Society for Microbiology
Citation: Applied and Environmental Microbiology 73(21): 6757-6767 (2007)
Abstract: Bile salts are natural detergents that facilitate the digestion and absorption of the hydrophobic components of the diet. However, their amphiphilic nature makes them very inhibitory for bacteria and strongly influences bacterial survival in the gastrointestinal tract. Adaptation to and tolerance of bile stress is therefore crucial for the persistence of bacteria in the human colonic niche. Bifidobacterium animalis subsp. lactis, a probiotic bacterium with documented health benefits, is applied largely in fermented dairy products. In this study, the effect of bile salts on proteomes of B. animalis subsp. lactis IPLA 4549 and its bile-resistant derivative B. animalis subsp. lactis 4549dOx was analyzed, leading to the identification of proteins which may represent the targets of bile salt response and adaptation in B. animalis subsp. lactis. The comparison of the wild-type and the bile-resistant strain responses allowed us to hypothesize about the resistance mechanisms acquired by the derivative resistant strain and about the bile salt response in B. animalis subsp. lactis. In addition, significant differences in the levels of metabolic end products of the bifid shunt and in the redox status of the cells were also detected, which correlate with some differences observed between the proteomes. These results indicate that adaptation and response to bile in B. animalis subsp. lactis involve several physiological mechanisms that are jointly dedicated to reduce the deleterious impact of bile on the cell's physiology.
URI: http://hdl.handle.net/10261/7048
ISSN: 0099-2240
DOI: 10.1128/AEM.00637-07
Appears in Collections:(IPLA) Artículos
Files in This Item:
File Description SizeFormat 
Borja bilis 6757.pdf586,25 kBAdobe PDFView/Open
Show full item record

WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.