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dc.contributor.authorCouté, Yohann-
dc.contributor.authorHernandez, Céline-
dc.contributor.authorRon D., Appel-
dc.contributor.authorSanchez, Jean-Charles-
dc.contributor.authorMargolles Barros, Abelardo-
dc.date.accessioned2008-09-01T12:02:38Z-
dc.date.available2008-09-01T12:02:38Z-
dc.date.issued2007-06-29-
dc.identifier.citationApplied and Environmental Microbiology 73(17): 5653-5656 (2007)en_US
dc.identifier.issn0099-2240-
dc.identifier.urihttp://hdl.handle.net/10261/7041-
dc.description.abstractStable isotope labeling of amino acids in cell culture was used for Bifidobacterium longum. A comprehensive proteomic strategy was developed and validated by designing an appropriate semidefined medium that allows stable replacement of natural leucine by [13C6]leucine. Using this strategy, proteins having variations of at least 50% in their expression rates can be quantified with great confidenceen_US
dc.description.sponsorshipThis work was financed by European Union FEDER funds and the Spanish Plan Nacional de I + D (project AGL2004-06727-C02-01/ALI and fellowship PR2006-0421) and by the Swiss National Science Foundation (grant 3100-A0-104214).en_US
dc.format.extent144719 bytes-
dc.format.mimetypeapplication/pdf-
dc.language.isoengen_US
dc.publisherAmerican Society for Microbiologyen_US
dc.rightsopenAccessen_US
dc.subjectBifidobacteriasen_US
dc.subjectLeucinaen_US
dc.titleLabeling of Bifidobacterium longum Cells with 13C-Substituted Leucine for Quantitative Proteomic Analysesen_US
dc.typeartículoen_US
dc.identifier.doi10.1128/AEM.00667-07-
dc.description.peerreviewedPeer revieweden_US
dc.contributor.funderEuropean Commission-
dc.contributor.funderComisión Interministerial de Ciencia y Tecnología, CICYT (España)-
dc.contributor.funderSwiss National Science Foundation-
dc.identifier.funderhttp://dx.doi.org/10.13039/501100000780es_ES
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