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Title

Transient activation of the c-Jun N-terminal kinase (JNK) activity by ligation of the tetraspan CD53 antigen in different cell types

AuthorsYunta, M.; Oliva, José Luis; Barcia, R.; Lazo, Pedro A. CSIC ORCID
KeywordsCD53
JNK
Tetraspanin
Signal transduction
Issue Date2002
PublisherWiley-Blackwell
CitationEuropean Journal of Biochemistry 269(3): 1012-1021 (2002)
AbstractTheCD53 antigen is amember of the tetraspaninmembrane protein family that is expressed in the lymphoid-myeloid lineage.We have studied the implication of CD53 antigen in signal transduction by determining the effect of its ligation on the c-Jun N-terminal kinase (JNK) in different cell types. Ligation of the rat or human CD53 antigen induces a three- to fourfold transient activation of JNKactivity that peaks at 3±5 min.The effect was detected by assaying the endogenous or exogenous (transfected) JNKactivity. The JNK response was detected in IR938F cells, a rat B-cell lymphoma, and in Jurkat cells derived from a human T-cell lymphoma. This JNK activation was not mediated by the vav oncogene, and CD53 does not cooperate with CD3 for vav activation. Asimilar JNKactivation was also detected in a human renal carcinoma cell line that was transiently transfected with the human CD53 cDNA to mimic the CD53 ectopic expression in carcinomas. In stable CD53-transfected cells it stimulated Jun-dependent transcriptional activity. We conclude that parts of the cell responses modulated by the CD53 are mediated by JNK activation, and this activation is independent of the different protein interactions that the CD53 protein has on speci®c cell types.
URIhttp://hdl.handle.net/10261/7017
DOI10.1046/j.0014-2956.2001.02741.x
ISSN0014-2956
Appears in Collections:(IBMCC) Artículos




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