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Antibodies raised against the N-terminal sequence of δ opioid receptors blocked δ-mediated supraspinal antinociception in mice

AuthorsGarzón, Javier ; Castro, M. Ángeles; Juarros, J. L.; Sánchez-Blázquez, Pilar
Issue Date1994
CitationLife Sciences 54: 191- 196 (1994)
AbstractA polyclonal antiserum directed against the first 16 aminoacids of the N- terminal sequence of the murine δ opioid receptor was raised in rabbits. The intracerebroventricular (i.c.v.) injection to mice of the anti δ receptor IgGs impaired the antinociception produced by DPDPE, [D-Ala2]-Deltorphin II, DADLE and β-endorphin-(1-31) when studied 24 h later in the tail-flick test. Antinociception produced by morphine and DAMGO was fully expressed in mice undergoing this treatment. The selective δ antagonist ICI 174864 (0.8 nmols/mouse, i.c.v.) significantly reduced the antinociceptive activity of opioids to the extent observed after giving the antibodies. ICI 174864 did not decrease further the antinociception that remained after the anti δ receptor serum. The specific binding displayed by 3 nM [3H]-DPDPE was reduced in membranes pre-incubated with the antiserum, whereas no change could be detected for 0.6 nM [3H]-DAMGO labelling μ receptors. This experimental approach revealed the δ component of opioid-evoked supraspinal antinociception in mice.
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