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Bcl-2 family gene modulation during spontaneous apoptosis of B-chronic lymphocytic leukemia cells

AuthorsSanz, Laura; García-Marco, José A.; Casanova, Benito; Fuente, María Teresa de la; Garcia-Gila, Mercedes; García-Pardo, Angeles ; Silva, Augusto
Bcl-2 family
mRNA expression
Issue Date12-Mar-2004
CitationBiochemical and Biophysical Research Communications 315(3):562-567(2004)
AbstractMalignant cell accumulation in B-cell chronic lymphocytic leukemia (B-CLL) is primarily caused by defective apoptosis rather than increased proliferation. To further understand the role of Bcl-2 family members, known regulators of apoptosis, in the abnormal B-CLL survival, we have measured their mRNA levels in fresh B-CLL cells and in cultures undergoing spontaneous apoptosis. Using RNA protection assays we found constitutive expression of most bcl-2 members with high levels of bcl2, bcl-w, bad, bak, bax, and the bcl-2/bax ratio, compared to normal PBL. Spontaneous apoptosis of B-CLL cells by in vitro culture resulted in decreased bcl-2, bcl-w, bfl-1, mcl-1, bak, bax, and bcl-2/bax expression. The pro-apoptotic member bik was only expressed in 5/19 cases and was not modulated during apoptosis, suggesting that bik is not involved in this process. Thus, several Bcl-2 family genes are regulated during B-CLL spontaneous apoptosis and their relative levels may contribute to in vivo progression of the disease
Description6 páginas, 3 figuras, 1 tabla -- PAGS nros. 562-567
Publisher version (URL)http://dx.doi.org/10.1016/j.bbrc.2004.01.095
Appears in Collections:(CIB) Artículos
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