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Amylase production by Aspergillus oryzae in a solid-state bioreactor with fed-batch operation using mussel processing wastewaters as feeding medium

AuthorsTorrado, Ana ; Vázquez, José Antonio ; Prieto, Miguel Ángel ; Fuciños, P. ; Montemayor, María I. ; Pastrana, Lorenzo ; González Fernández, Pilar ; Murado García, Miguel Anxo
KeywordsAmylase production
Mussel processing wastewaters
Solid-state bioreactor
Weibull equation
Issue Date2013
PublisherJohn Wiley & Sons
CitationJournal of Chemical Technology and Biotechnology 88(2): 226-236 (2013)
AbstractBACKGROUND: The high oxygen availability in solid-state cultures makes them especially suitable for fungal enzyme production. Glycogen-rich mussel processing wastewaters have been used successfully as substrates for amylase production by Aspegillus oryzae in solid-state cultures supported in polyurethane foam. The aim of this work was to study the fed-batch mode in a scalable solid-state bioreactor to extend the productive period and obtain high amylase production. Culture salinization due to the NaCl content of these wastewaters is the main drawback. RESULTS: Evaporation of the excess liquid added during feeding led to progressive salt accumulation. The effect of culture salinization on amylase production was analysed and mathematically modelled, and the IC50 (65.4 g L−1 of NaCl) was calculated. An optimum operation mode for this bioreactor was designed that included foam extrusion for removing the incubated medium, washing and a final recharge with fresh medium every 72 h of incubation. This procedure kept the salt concentration under IC50 and increased the amylase production from 3000 to 12 000 UE g−1. CONCLUSION: An operating mode with intermittent extrusions and washings of the support between feedings was found to be an appropriate procedure for preventing the accumulation of inhibitory compounds in fed-batch solid-state cultures. © 2012 Society of Chemical Industry
Description11 páginas, 6 figuras, 4 tablas
Publisher version (URL)http://dx.doi.org/10.1002/jctb.3813
Appears in Collections:(IIM) Artículos
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