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Title

p38 MAPK protects against TNF-alpha-provoked apoptosis in LNCap prostatic cells

AuthorsRicote, Mónica; García-Tuñón, Ignacio; Fraile, Benito; Fernández, Carlos F. González; Aller, Patricio ; Paniagua, Ricardo; Royuela, Mar
KeywordsApoptosis
Protate
Cancer
LNCap
PC3
P38
TNF
Issue DateNov-2006
PublisherSpringer
CitationApoptosis 11(11):1969-1975(2006)
AbstractPURPOSE: One of the most relevant aspects in cell death regulation is the signalling of apoptosis by the serine/threonine kinases MAPKs. The aim of this study was to investigate the effects of TNF-alpha stimulation on MAPK activation, and the pro- or anti-apoptotic role of these kinases in LNCaP and PC3 cells. MATERIAL AND METHODS: Treatments were carried out using several TNF-alpha concentrations, as well as specific pharmacological inhibitors of MAPKs. Apoptosis rates were evaluated by DAPI staining and flow cytometry. MAPK phosphorylation/activation was measured by Western blot. RESULTS: TNF-alpha induced apoptosis in a dose-dependent manner in LNCaP but not in PC3 cells. The MAPK inhibitors revealed that the apoptotic rate in LNCaP cells increased significantly following p38 inhibition. The kinase inhibitors failed to cause changes in apoptosis in PC3 cells. CONCLUSIONS: The potentiation of apoptosis by p38 inhibition points to this kinase as a possible target for the treatment of androgen-dependent prostatic cancer
Description7 páginas, 4 figuras -- PAGS nros. 1969-1975
Publisher version (URL)http://dx.doi.org/10.1007/s10495-006-0086-9
URIhttp://hdl.handle.net/10261/66687
DOI10.1007/s10495-006-0086-9
ISSN1360-8185
E-ISSN1573-675X
Appears in Collections:(CIB) Artículos
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