English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/66492
Share/Impact:
Statistics
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:
Title

Genes of the ecdysone biosynthesis pathway are regulated by the dATAC histone acetyltransferase complex in Drosophila

AuthorsPankotai, Tibor; Popescu, Cristina; Martín Casacuberta, David A. ; Grau, Benjamín ; Zsindely, N.; Bodai, L.; Tora, Laszlo; Ferrús, Alberto ; Boros, I.
Issue DateSep-2010
PublisherAmerican Society for Microbiology
CitationMolecular and Cellular Biology 30(17): 4254-4266 (2010)
AbstractUncovering mechanisms that regulate ecdysone production is an important step toward understanding the regulation of insect metamorphosis and processes in steroid-related pathologies. We report here the transcriptome analysis of Drosophila melanogaster dAda2a and dAda3 mutants, in which subunits of the ATAC acetyltransferase complex are affected. In agreement with the fact that these mutations lead to lethality at the start of metamorphosis, both the ecdysone levels and the ecdysone receptor binding to polytene chromosomes are reduced in these flies. The cytochrome genes (spookier, phantom, disembodied, and shadow) involved in steroid conversion in the ring gland are downregulated, while the gene shade, which is involved in converting ecdysone into its active form in the periphery, is upregulated in these dATAC subunit mutants. Moreover, driven expression of dAda3 at the site of ecdysone synthesis partially rescues dAda3 mutants. Mutants of dAda2b, a subunit of the dSAGA histone acetyltransferase complex, do not share phenotype characteristics and RNA profile alterations with dAda2a mutants, indicating that the ecdysone biosynthesis genes are regulated by dATAC, but not by dSAGA. Thus, we provide one of the first examples of the coordinated regulation of a functionally linked set of genes by the metazoan-specific ATAC complex. Copyright © 2010, American Society for Microbiology. All Rights Reserved.
Publisher version (URL)http://dx.doi.org/10.1128/MCB.00142-10
URIhttp://hdl.handle.net/10261/66492
DOI10.1128/MCB.00142-10
E-ISSN1098-5549
Identifiersdoi: 10.1128/MCB.00142-10
issn: 0270-7306
Appears in Collections:(IC) Artículos
(IBE) Artículos
Files in This Item:
File Description SizeFormat 
accesoRestringido.pdf15,38 kBAdobe PDFThumbnail
View/Open
Show full item record
Review this work
 

Related articles:


WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.