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Determination of palytoxins in samples from ostreopsis outbreaks in Llavaneres (NW Mediterranean coasts)

AuthorsRiobó, Pilar ; Franco, José M. ; Bravo, Isabel; Fraga, Santiago; Ramilo, Isabel; Rial, Pilar; Rodríguez, Francisco; Sala, M. Montserrat ; Vila, Magda
Issue Date2012
PublisherIntergovernmental Oceanographic Commission
International Society for the Study of Harmful Algae
CitationProceedings of the 14th International Conference on Harmful Algae : 33-35 (2012)
AbstractThe objective of the present work was to link Ostreopsis blooms with respiratory problems. To test this hypothesis, samples of seawater, macroalgae, benthic marine invertebrates and aerosols were collected during 2009 and 2010 in the framework of the EBITOX project. Different extraction procedures have been used depending on sample type. The extracts were analysed by haemolytic assay (Riobó et al 2008) and liquid chromatography with fluorescence detection (LC–FLD) (Riobó et al 2006) and the presence of palytoxin was confirmed by liquid chromatography coupled with mass spectrometry (LC-MS) (Riobó et al 2006). Strains isolated from Llavaneres were cultured in the laboratory and analysed by the same chemical and biological methods. Toxins from field samples and cultures were compared. Presence of palytoxins in macroalgae and cultures was confirmed but it has not yet been detected in aerosol filters or dissolved in seawater. Therefore, the hypothesis remains unresolved.
Description14th International Conference on Harmful Algae, 1-5 November 2010, Creta, Greece.-- 3 pages, 1 figure.
Publisher version (URL)http://www.issha.org/Welcome-to-ISSHA/Conferences/ICHA-conference-proceedings/ICHA14-Proceedings
Appears in Collections:(IIM) Libros y partes de libros
(ICM) Libros y partes de libros
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