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Title

Isolation and bioenergetic characterization of mitochondria from Pichia pastoris

AuthorsGonzález-Barroso, M. Mar ; Ledesma, Amalia ; Lepper, Simone; Pérez-Magán, Elisa; Zaragoza, Pilar ; Rial, Eduardo
KeywordsMitochondria
Pichia pastoris
yeast
mitochondrial respiration
Issue DateMar-2006
PublisherJohn Wiley & Sons
CitationYeast, 23 (4) : 307-313 (2006)
AbstractPichia pastoris is a methylotrophic yeast of high biotechnological interest. The bioenergetic properties of mitochondria from Pichia pastoris have not yet been determined. We report on a protocol for the isolation of the mitochondria in a state that shows good energy coupling. Analysis of Pichia pastoris growth and bioenergetic properties of the isolated mitochondria reveals that glycerol is the carbon source that yields the best results. Under our growth conditions, mitochondria oxidize external NADH but do not possess an alternative oxidase. Finally, Pichia pastoris mitochondria also lack the nucleotide-stimulated uncoupling pathway previously identified in Saccharomyces cerevisiae
Description7 páginas, 2 figuras, 1 tabla -- PAGS nros. 307-313
Publisher version (URL)http://dx.doi.org/10.1002/yea.1355
URIhttp://hdl.handle.net/10261/66306
DOI10.1002/yea.1355
ISSN0749-503X
E-ISSN1097-0061
Appears in Collections:(CIB) Artículos
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